Ferric reducing anti-oxidant power assay in plant extract

The ferric reducing anti-oxidant power (FRAP) assay involved the following steps:  a) preparation of samples, b) reactions and c) finally measuring absorbance of sample and standard at 700 nm using spectrophotometer. The samples were methanolic extract, different fractions (n-hexane, chloroform) and...

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Bibliographic Details
Main Authors: Maruthamuthu Vijayalakshmi (Author), Kandasamy Ruckmani (Author)
Format: Book
Published: Bangladesh Pharmacological Society, 2016-06-01T00:00:00Z.
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Summary:The ferric reducing anti-oxidant power (FRAP) assay involved the following steps:  a) preparation of samples, b) reactions and c) finally measuring absorbance of sample and standard at 700 nm using spectrophotometer. The samples were methanolic extract, different fractions (n-hexane, chloroform) and standard ascorbic acid. The solutions prepared were buffer solution (pH 6.6), 1% potassium ferricyanide solution, 10% trichloroacetic acid and 0.1% ferric chloride.  Video Clip of Methodology: Ferric reducing anti-oxidant power assay: 21 min 18 sec   Full Screen   Alternate
Item Description:10.3329/bjp.v11i3.27663
1991-0088