Antibiotic Drug Resistance Pattern of Uropathogens in Pediatric Patients in Pakistani Population

• Main Authors: Zakia Iqbal (Author), Ahsan Sattar Sheikh (Author), Anwaar Basheer (Author), Hadiqa tul Hafsa (Author), Mehboob Ahmed (Author), Anjum Nasim Sabri (Author), Samiah Shahid (Author)
• Format: Book
• Published: MDPI AG, 2023-02-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: 10.3390/antibiotics12020395; 2079-6382
• Keywords: uropathogens; <i>Escherichia</i>; <i>Klebsiella</i>; antibiotic resistance; Therapeutics. Pharmacology; RM1-950
• Source: Antibiotics, Vol 12, Iss 2, p 395 (2023)

The common prevalent diseases in the age of 0 to 6 are related to urinary tract infections. If not properly diagnosed, they will lead to urological and nephrological complications. Uropathogens are developing resistance against most drugs and are harder to treat. A study was done on the inpatients and outpatients of the two hospitals located in Lahore. A total of 39,750 samples that were both male and female were collected. <i>Escherichia</i> and <i>Klebsiella</i> were found in 234 samples based on biochemical characterization, growth on CLED agar, and white blood cell/pus cell (WBC) microscopy. In comparison to males, female samples had a higher number of uropathogens (1:1.29). From the samples of Shaikh Zayed Hospital (SZH), the ratio of <i>Klebsiella</i> to <i>Escherichia</i> (1:1.93) was reported, while this ratio was 1.84:1 from the Children Hospital (CH). The incidence of UTI was higher in the month of September. Randomly selected <i>Escherichia</i> and <i>Klebsiella</i> were verified via a 16S rRNA sequence. Antibiotic resistance profiling of isolated bacterial strains was done against 23 antibiotics. The most efficient antibiotics against <i>Klebsiella</i> and <i>Escherichia</i> were colistin sulphate (100% sensitivity against bacteria from CH; 99.3% against strains from SZH) and polymyxin B (100% sensitivity against strains from SZH; 98.8% against strains from CH). Sensitivity of the total tested strains against meropenem (74%, SZH; 70% CH), Fosfomycin (68%, SZH; 73% CH strains), amikacin (74% SZH; 55% CH), and nitrofurantoin (71% SZH;67% CH) was found, Amoxicillin, ampicillin, and cefuroxime showed 100 to ≥90% resistance and are the least effective.