Green Synthesis of Characterized Silver Nanoparticle Using <i>Cullen tomentosum</i> and Assessment of Its Antibacterial Activity
Plants serve as an important source of medicine and provide suitable candidate compounds to produce eco-friendly therapeutic agents. They also represent a source of bio-reducer and stabilizer for the development of nanoparticles for downstream applications. This study focused on the green synthesis...
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| Main Authors: | , , , , , |
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| Format: | Book |
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MDPI AG,
2023-01-01T00:00:00Z.
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| Summary: | Plants serve as an important source of medicine and provide suitable candidate compounds to produce eco-friendly therapeutic agents. They also represent a source of bio-reducer and stabilizer for the development of nanoparticles for downstream applications. This study focused on the green synthesis of silver nanoparticle (CTAgNP) using <i>Cullen tomentosum</i> (Thunb.) J.W. Grimes acetone extract and the evaluation of the antibacterial activity of the plant extract and biogenic nanoparticles against two Gram-positive bacteria strains, namely <i>Bacillus cereus</i> and <i>Staphylococcus aureus</i>. In addition, the phytochemical profile of <i>C</i>. <i>tomentosum</i> was established using liquid chromatography-mass spectrometry (LC-MS). The antibacterial effect of the extract and CTAgNP was moderate based on the minimum inhibitory concentration (MIC) values obtained. The MIC values of 2.6 mg/mL and 3.1 mg/mL were recorded for <i>C</i>. <i>tomentosum</i> extract against <i>B</i>. <i>cereus</i> and <i>S</i>. <i>aureus</i>, respectively. On the other hand, the CTAgNP had MIC values of 1.5 mg/mL and 2.6 mg/mL against <i>B. cereus</i> and <i>S. aureus</i>, respectively. The nanoparticle exhibited surface charge of −37 ± 7.67 mV and average hydro-dynamic size of 145 nm. X-ray diffraction illustrates that metallic nanoparticles were formed and had a face-centered cubic structure. Microscopic and spectroscopic techniques revealed that the CTAgNP was covered by a protective shell layer constituted of organic compounds originating from the plant extract. The acetone extract of <i>C</i>. <i>tomentosum</i> could be useful to the bio-pharma industries in the large-scale manufacture of nanoparticle-based medications to fight against microbes that constitute a threat to the survival of humanity. |
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| Item Description: | 10.3390/antibiotics12020203 2079-6382 |