<i>Ginkgo biloba</i> Extract Stimulates Adipogenesis in 3T3-L1 Preadipocytes

• Main Authors: Fernanda Malanconi Thomaz (Author), Jussara de Jesus Simão (Author), Viviane Simões da Silva (Author), Meira Maria Forcelini Machado (Author), Lila Missae Oyama (Author), Eliane Beraldi Ribeiro (Author), Maria Isabel Cardoso Alonso Vale (Author), Monica Marques Telles (Author)
• Format: Book
• Published: MDPI AG, 2022-10-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: 10.3390/ph15101294; 1424-8247
• Keywords: adipocytes hyperplasia; natural compounds; preadipocytes maturation; polyphenols; pro-adipogenesis effect; Medicine; R; Pharmacy and materia medica; RS1-441
• Source: Pharmaceuticals, Vol 15, Iss 10, p 1294 (2022)

Smaller adipocytes are related to the reversal of metabolic disorders, suggesting that molecules that can act in the adipogenesis pathway are of great interest. The objective of this study was to investigate the effect of <i>Ginkgo biloba</i> extract (GbE) in modulating the differentiation in preadipocytes. 3T3-L1 preadipocytes were differentiated for 7 days into adipocytes without (control group) and with GbE at 1.0 mg/mL. Lipid content and gene expression were analyzed on day 7 (D7) by Oil Red O staining and PCR Array Gene Expression. Western blotting analysis of the key adipogenesis markers was evaluated during the differentiation process at days 3 (D3), 5 (D5), and 7 (D7). GbE increased lipid content and raised the gene expression of the main adipogenesis markers. Key proteins of the differentiation process were modulated by GbE, since C/EBPβ levels were decreased, while C/EBPα levels were increased at D7. Regarding the mature adipocytes' markers, GbE enhanced the levels of both FABP4 at D5, and perilipin at D3 and D5. In summary, the present findings showed that GbE modulated the adipogenesis pathway suggesting that the treatment could accelerate the preadipocyte maturation, stimulating the expression of mature adipocyte proteins earlier than expected.