Ethyl Acetate Fraction of <i>Bixa orellana</i> and Its Component Ellagic Acid Exert Antibacterial and Anti-Inflammatory Properties against <i>Mycobacterium abscessus</i> subsp. <i>massiliense</i>
<i>Mycobacterium abscessus</i> subsp. <i>massiliense</i> (<i>Mabs</i>) causes chronic infections, which has led to the need for new antimycobacterial agents. In this study, we investigated the antimycobacterial and anti-inflammatory activities of the ethyl acetate...
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Main Authors: | , , , , , , , , , , , , , , , , |
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Format: | Book |
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MDPI AG,
2022-06-01T00:00:00Z.
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Summary: | <i>Mycobacterium abscessus</i> subsp. <i>massiliense</i> (<i>Mabs</i>) causes chronic infections, which has led to the need for new antimycobacterial agents. In this study, we investigated the antimycobacterial and anti-inflammatory activities of the ethyl acetate fraction of <i>Bixa orellana</i> leaves (BoEA) and ellagic acid (ElAc). In silico analysis predicted that ElAc had low toxicity, was not mutagenic or carcinogenic, and had antimicrobial and anti-inflammatory activities. Apparently, ElAc can interact with COX2 and Dihydrofolate reductase (DHFR) enzymes, which could explain both activities. In vitro analysis showed that BoEA and ElAc exerted antimicrobial activity against <i>Mabs</i> (minimum inhibitory concentration of 1.56, 1.56 mg/mL and bactericidal concentration of 6.25, 3.12 mg/mL, respectively. Clarithromycin showed MIC and MBC of 1 and 6 µg/mL). Treatment with BoEA or ElAc increased survival of <i>Tenebrio molitor</i> larvae after lethal infection with <i>Mabs</i> and reduced carrageenan-induced paw edema in mice, around 40% of edema volume after the fourth hour, similarly to diclofenac. In conclusion, BoEA and ElAc exert antimicrobial effects against <i>Mabs</i> and have anti-inflammatory effects, making them potential sources of antimycobacterial drugs. The biological activities of ElAc may be due to its high binding affinities predicted for COX2 and DHFR enzymes. |
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Item Description: | 10.3390/antibiotics11060817 2079-6382 |