Ethyl Acetate Fraction of <i>Bixa orellana</i> and Its Component Ellagic Acid Exert Antibacterial and Anti-Inflammatory Properties against <i>Mycobacterium abscessus</i> subsp. <i>massiliense</i>

• Main Authors: Roberval Nascimento Moraes-Neto (Author), Gabrielle Guedes Coutinho (Author), Ana Caroline Santos Ataíde (Author), Aline de Oliveira Rezende (Author), Camila Evangelista Carnib Nascimento (Author), Rafaela Pontes de Albuquerque (Author), Cláudia Quintino da Rocha (Author), Adriana Sousa Rêgo (Author), Maria do Socorro de Sousa Cartágenes (Author), Ana Lúcia Abreu-Silva (Author), Igor Victor Ferreira dos Santos (Author), Cleydson Breno Rodrigues dos Santos (Author), Rosane Nassar Meireles Guerra (Author), Rachel Melo Ribeiro (Author), Valério Monteiro-Neto (Author), Eduardo Martins de Sousa (Author), Rafael Cardoso Carvalho (Author)
• Format: Book
• Published: MDPI AG, 2022-06-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

<i>Mycobacterium abscessus</i> subsp. <i>massiliense</i> (<i>Mabs</i>) causes chronic infections, which has led to the need for new antimycobacterial agents. In this study, we investigated the antimycobacterial and anti-inflammatory activities of the ethyl acetate fraction of <i>Bixa orellana</i> leaves (BoEA) and ellagic acid (ElAc). In silico analysis predicted that ElAc had low toxicity, was not mutagenic or carcinogenic, and had antimicrobial and anti-inflammatory activities. Apparently, ElAc can interact with COX2 and Dihydrofolate reductase (DHFR) enzymes, which could explain both activities. In vitro analysis showed that BoEA and ElAc exerted antimicrobial activity against <i>Mabs</i> (minimum inhibitory concentration of 1.56, 1.56 mg/mL and bactericidal concentration of 6.25, 3.12 mg/mL, respectively. Clarithromycin showed MIC and MBC of 1 and 6 µg/mL). Treatment with BoEA or ElAc increased survival of <i>Tenebrio molitor</i> larvae after lethal infection with <i>Mabs</i> and reduced carrageenan-induced paw edema in mice, around 40% of edema volume after the fourth hour, similarly to diclofenac. In conclusion, BoEA and ElAc exert antimicrobial effects against <i>Mabs</i> and have anti-inflammatory effects, making them potential sources of antimycobacterial drugs. The biological activities of ElAc may be due to its high binding affinities predicted for COX2 and DHFR enzymes.

Item Description

10.3390/antibiotics11060817
2079-6382