Denture-Soaking Solution Containing <i>Piper betle</i> Extract-Loaded Polymeric Micelles; Inhibition of <i>Candida albicans</i>, Clinical Study, and Effects on Denture Base Resin

• Main Authors: Siriporn Okonogi (Author), Pimpak Phumat (Author), Sakornrat Khongkhunthian (Author), Kullapop Suttiat (Author), Pisaisit Chaijareenont (Author)
• Format: Book
• Published: MDPI AG, 2021-04-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

<i>Candida albicans</i> is a common overgrowth in people wearing dentures. Long-term use of antifungal chemicals carries a risk of toxic side effects. This study focused on the edible <i>Piper betle</i> extract because of its safety. The broth dilution method was applied for antifungal determination of the ethyl acetate fractionated extract (fEA) and fEA-loaded polymeric micelles (PMF). The PMF was prepared by thin-film hydration using poloxamer 407 as a polymer base. The results found that the weight ratio of fEA to polymer is the main factor to obtain PMF system as a clear solution, nanoparticle sizes, narrow size distribution, negative zeta potential, and high entrapment efficiency. The activity of PMF against <i>C. albicans</i> is significantly higher than fEA alone, with a minimum fungicidal concentration of 1.5 mg/mL. PMF from 1:3 ratio of fEA to polymer is used to develop a denture-soaking solution contained 1.5 mg fEA/mL (PMFS). A clinical study on dentures of 15 volunteers demonstrated an 86.1 ± 9.2% reduction of <i>C. albicans</i> after soaking the dentures in PMFS daily for 14 days. Interestingly, PMFS did not change the hardness and roughness of the denture base resins. The developed PMFS may serve as a potential natural denture-soaking solution against candidiasis in denture wearers.

Item Description

10.3390/antibiotics10040440
2079-6382