p53-protein over-expression and gene mutational of oral carcinoma in-situ

• Main Authors: Mei Syafriadi (Author), Takashi Saku (Author)
• Format: Book
• Published: Universitas Airlangga, 2007-06-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

<span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>We had been reported histological types of oral carcinoma in-situ (oral CIS), such as basaloid, verrucous, and acanthothic/ </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>atrophic types. We considered that they have different histological appearance influenced by molecular behavior. To understand the </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>molecular behavior of them we examined p53 exon 4-8 gene mutation and their protein expression</em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #ee1f23; font-style: normal; font-variant: normal;"><em>. </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Using 35 cases formalin-fixed </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>paraffin sections of oral CIS and 10 cases of mild and moderate squamous epithelial displasia (SED) as a control were subjected to </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>p53 immunohistochemistry. In the next step all cases were subjected to p53 gene mutations analysis by laser capturing microdissection </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>and direct sequencing of PCR product for exon 4-8. Showed that p53-protein over-expression were found in basal layer of SED and </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>the p53 protein over-expression were confined in the whole layer of CIS-basaloid type, basal and parabasal layers of CIS-verrucous </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>type, and sporadically in the basal layer of CIS-acanthothic type. Mutational analysis for p53 gene showed 43% of total cases of CIS </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>had p53 gene mutation therefore CIS-basaloid type had mutations more frequently than the other types and mutation in exon 8 more </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>dominant than other exons, which had some common mutation at codons 196, 248, 282, 291, and 306, while no particular mutations </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>were found in control (SED)</em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #ee1f23; font-style: normal; font-variant: normal;"><em>.</em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Our criteria to diagnose several types of oral carcinoma in-situ by p53 protein expression and mutational </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>analysis could be used to understand molecular behavior of CIS.</em></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span>

Item Description

1978-3728
2442-9740
10.20473/j.djmkg.v40.i2.p55-60