Effect of crude alcohol extracts on some parts of clover plant Dianthus caryophllus in inhibition of fungal growth Fusarium culum and Fusarium oxysporum

• Main Authors: Khazal Ameen (Author), Firass Khudhair (Author)
• Format: Book
• Published: College of Education for Pure Sciences, 2007-09-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: 1812-125X; 2664-2530; 10.33899/edusj.2007.162830
• Keywords: crude alcohol; clover plant; fusarium oxysporum; Education; L; Science (General); Q1-390
• Source: مجلة التربية والعلم, Vol 20, Iss 1, Pp 443-453 (2007)

Callus has been derived from Carnation leaves, on (MS) medium, Containing Various Concentrations of plant growth regulators, 0.1 mg/L BA and 0.5 mg/L 2,4-D. Established callus showed a good ability for<br />differentiation and shoot formation on MS medium supplemented with 3.0 mg/L BA and 0.1 mg/L IAA at a rate of 100%. Different rooting concentrations were used with IBA in MS medium, 100% rooting was<br />obtained when IBA used at 1.0 mg/L. Phenols were separated from crude alcoholic extractions from leaves of seed plants, callus of leaves and leaves derived from regeneration plants. Phenols were identified by using ferric chloride reagent. Treatment of both Fusarium oxysporum and Fusarium Culum with these extractions was successful in retarding the growth of both fungi, by means of dry weight. The best crude<br />alcoholic extract in retarding the growth of both fungi was found to be the callus of leaves extracts which caused 67.7% and 62.1 retardation in growth of both fungi Fusarium oxysporum and Fusarium culum respectively.