ANALISIS KUANTITATIF ISOFLAVON TEMPE SECARA CEPAT DAN SEDERHANA MENGGUNAKAN METODE KROMATOGRAFI LAPIS TIPIS- DENSITOMETRI

• Main Authors: Agustina Setiawati (Author), Sri Hartati Yuliani (Author), Enade Perdana Istyastono (Author), Michael Rahardja Gani (Author), Evy Fenny Veronica (Author), Dina Christin Ayuning Putri (Author), Reza Eka Putra (Author), David Chandra Putra (Author), Agnes Mutiara Kurniawan (Author)
• Format: Book
• Published: Universitas Sanata Dharma, 2014-05-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

Abstract: Rapid and simple quantitative analysis isoflavones tempe using densitometric TLC has been done. The mobile phase of the system was chloroform: methanol: ethylacetate (45: 5: 0.75). Thin layer chromatography was performed on aluminium TLC plates.Ascending distance of 1 μL sample was performanced 10 cm. Then the plate was scanned at 261 nm. A linear relationship obtained at 0.08 - 2 μg/spot with r= 0.9986. The LOD and LOQ of isoflavone were 0.014 μg/spot and 0.048 μg/spot. Genistein contained in tempe was 0.151± 0.005 % b/b. Keywords: tempe, isoflavon, TLC

Item Description

1693-5683
2527-7146
10.24071/jpsc.11180