Development and validation of RP-HPLC method for estimation of eplerenone in spiked human plasma

A rapid and simple high performance liquid chromatography (HPLC) method with a UV detection (241 nm) was developed and validated for estimation of eplerenone from spiked human plasma. The analyte and the internal standard (valdecoxib) were extracted with a mixture of dichloromethane and diethyl eth...

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Bibliographic Details
Main Authors: Paraag Gide (Author), Sandeep Sonawane (Author), Abhishek Chitnis (Author)
Format: Book
Published: Elsevier, 2012-10-01T00:00:00Z.
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Summary:A rapid and simple high performance liquid chromatography (HPLC) method with a UV detection (241 nm) was developed and validated for estimation of eplerenone from spiked human plasma. The analyte and the internal standard (valdecoxib) were extracted with a mixture of dichloromethane and diethyl ether. The chromatographic separation was performed on a HiQSil C-18HS column (250 mmÃ4.6 mm, 5 μm) with a mobile phase consisting of acetonitrile:water (50:50, v/v) at flow rate of 1 mL/min. The calibration curve was linear in the range 100â3200 ng/mL and the heteroscedasticity was minimized by using weighted least squares regression with weighting factor 1/X. Keywords: Eplerenone, Liquidâliquid extraction, Weighted regression, HPLCâUV
Item Description:2095-1779
10.1016/j.jpha.2012.04.006