Development and validation of RP-HPLC method for estimation of eplerenone in spiked human plasma
A rapid and simple high performance liquid chromatography (HPLC) method with a UV detection (241Â nm) was developed and validated for estimation of eplerenone from spiked human plasma. The analyte and the internal standard (valdecoxib) were extracted with a mixture of dichloromethane and diethyl eth...
Saved in:
Main Authors: | , , |
---|---|
Format: | Book |
Published: |
Elsevier,
2012-10-01T00:00:00Z.
|
Subjects: | |
Online Access: | Connect to this object online. |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Summary: | A rapid and simple high performance liquid chromatography (HPLC) method with a UV detection (241 nm) was developed and validated for estimation of eplerenone from spiked human plasma. The analyte and the internal standard (valdecoxib) were extracted with a mixture of dichloromethane and diethyl ether. The chromatographic separation was performed on a HiQSil C-18HS column (250 mmÃ4.6 mm, 5 μm) with a mobile phase consisting of acetonitrile:water (50:50, v/v) at flow rate of 1 mL/min. The calibration curve was linear in the range 100â3200 ng/mL and the heteroscedasticity was minimized by using weighted least squares regression with weighting factor 1/X. Keywords: Eplerenone, Liquidâliquid extraction, Weighted regression, HPLCâUV |
---|---|
Item Description: | 2095-1779 10.1016/j.jpha.2012.04.006 |