Development and validation of RP-HPLC method for estimation of eplerenone in spiked human plasma
A rapid and simple high performance liquid chromatography (HPLC) method with a UV detection (241Â nm) was developed and validated for estimation of eplerenone from spiked human plasma. The analyte and the internal standard (valdecoxib) were extracted with a mixture of dichloromethane and diethyl eth...
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Elsevier,
2012-10-01T00:00:00Z.
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| LEADER | 00000 am a22000003u 4500 | ||
|---|---|---|---|
| 001 | doaj_b4dc7adc28b94ef5819bff79c3721bd5 | ||
| 042 | |a dc | ||
| 100 | 1 | 0 | |a Paraag Gide |e author |
| 700 | 1 | 0 | |a Sandeep Sonawane |e author |
| 700 | 1 | 0 | |a Abhishek Chitnis |e author |
| 245 | 0 | 0 | |a Development and validation of RP-HPLC method for estimation of eplerenone in spiked human plasma |
| 260 | |b Elsevier, |c 2012-10-01T00:00:00Z. | ||
| 500 | |a 2095-1779 | ||
| 500 | |a 10.1016/j.jpha.2012.04.006 | ||
| 520 | |a A rapid and simple high performance liquid chromatography (HPLC) method with a UV detection (241 nm) was developed and validated for estimation of eplerenone from spiked human plasma. The analyte and the internal standard (valdecoxib) were extracted with a mixture of dichloromethane and diethyl ether. The chromatographic separation was performed on a HiQSil C-18HS column (250 mmÃ4.6 mm, 5 μm) with a mobile phase consisting of acetonitrile:water (50:50, v/v) at flow rate of 1 mL/min. The calibration curve was linear in the range 100â3200 ng/mL and the heteroscedasticity was minimized by using weighted least squares regression with weighting factor 1/X. Keywords: Eplerenone, Liquidâliquid extraction, Weighted regression, HPLCâUV | ||
| 546 | |a EN | ||
| 690 | |a Therapeutics. Pharmacology | ||
| 690 | |a RM1-950 | ||
| 655 | 7 | |a article |2 local | |
| 786 | 0 | |n Journal of Pharmaceutical Analysis, Vol 2, Iss 5, Pp 390-393 (2012) | |
| 787 | 0 | |n http://www.sciencedirect.com/science/article/pii/S209517791200055X | |
| 787 | 0 | |n https://doaj.org/toc/2095-1779 | |
| 856 | 4 | 1 | |u https://doaj.org/article/b4dc7adc28b94ef5819bff79c3721bd5 |z Connect to this object online. |