In Silico Study of Superoxide Dismutase Gene Family in Potato and Effects of Elevated Temperature and Salicylic Acid on Gene Expression

• Main Authors: Jelena Rudić (Author), Milan B. Dragićević (Author), Ivana Momčilović (Author), Ana D. Simonović (Author), Danijel Pantelić (Author)
• Format: Book
• Published: MDPI AG, 2022-02-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: 10.3390/antiox11030488; 2076-3921
• Keywords: superoxide dismutase; potato; AlphaFold; heat stress; salicylic acid; Therapeutics. Pharmacology; RM1-950
• Source: Antioxidants, Vol 11, Iss 3, p 488 (2022)

Potato (<i>Solanum tuberosum</i> L.) is the most important vegetable crop globally and is very susceptible to high ambient temperatures. Since heat stress causes the accumulation of reactive oxygen species (ROS), investigations regarding major enzymatic components of the antioxidative system are of the essence. Superoxide dismutases (SODs) represent the first line of defense against ROS but detailed in silico analysis and characterization of the potato <i>SOD</i> gene family have not been performed thus far. We have analyzed eight functional <i>SOD</i> genes, three <i>StCuZnSOD</i>s, one <i>StMnSOD</i>, and four <i>StFeSOD</i>s, annotated in the updated version of potato genome (Spud DB DM v6.1). The <i>StSOD</i> genes and their respective proteins were analyzed in silico to determine the exon-intron organization, splice variants, cis-regulatory promoter elements, conserved domains, signals for subcellular targeting, 3D-structures, and phylogenetic relations. Quantitative PCR analysis revealed higher induction of <i>StCuZnSOD</i>s (the major potato <i>SOD</i>s) and <i>StFeSOD3</i> in thermotolerant cultivar Désirée than in thermosensitive Agria and Kennebec during long-term exposure to elevated temperature. <i>StMnSOD</i> was constitutively expressed, while expression of <i>StFeSOD</i>s was cultivar-dependent. The effects of salicylic acid (10⁻⁵ M) on <i>StSOD</i>s expression were minor. Our results provide the basis for further research on <i>StSOD</i>s and their regulation in potato, particularly in response to elevated temperatures.