Antimicrobial, Antioxidant, and Cytotoxic Activities of <i>Juglans regia</i> L. Pellicle Extract
The difficulty to treat resistant strains-related hospital-acquired infections (HAIs) promoted the study of phytoextracts, known sources of bioactive molecules. Accordingly, in the present study, the pharmacological activities of <i>Juglans regia</i> (L.) pellicle extract (WPE) were inve...
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| Main Authors: | , , , , , , , , , , |
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| Format: | Book |
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MDPI AG,
2021-02-01T00:00:00Z.
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| Summary: | The difficulty to treat resistant strains-related hospital-acquired infections (HAIs) promoted the study of phytoextracts, known sources of bioactive molecules. Accordingly, in the present study, the pharmacological activities of <i>Juglans regia</i> (L.) pellicle extract (WPE) were investigated. The antiviral effect was tested against <i>Herpes simplex</i> virus type 1 and 2, <i>Poliovirus</i> 1, <i>Adenovirus</i> 2, <i>Echovirus</i> 9, <i>Coxsackievirus</i> B1 through the plaque reduction assay. The antibacterial and antifungal activities were evaluated against medically important strains, by the microdilution method. DPPH and superoxide dismutase (SOD)s-like activity assays were used to determine the antioxidant effect. Besides, the extract was screened for cytotoxicity on Caco-2, MCF-7, and HFF1 cell lines by the 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay. The total phenolic and flavonoid contents were also evaluated. Interestingly, WPE inhibited <i>Herpes simplex</i> viruses (HSVs) replication, bacterial and fungal growth. WPE showed free radical scavenging capacity and inhibited superoxide anion formation in a dose-dependent manner. These effects could be attributed to the high content of phenols and flavonoids, which were 0.377 ± 0.01 mg GE/g and 0.292 ± 0.08 mg CE/g, respectively. Moreover, WPE was able to reduce Caco-2 cell viability, at both 48 h and 72 h. The promising results encourage further studies aimed to better elucidate the role of WPE in the prevention of human infectious diseases. |
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| Item Description: | 10.3390/antibiotics10020159 2079-6382 |