Disclosing the Antioxidant and Neuroprotective Activity of an Anthocyanin-Rich Extract from Sweet Cherry (<i>Prunus avium</i> L.) Using In Vitro and In Vivo Models
In this study, an autochthonous variety of sweet cherry (<i>Prunus avium</i> L.), namely "Moretta di Vignola", was processed to prepare extracts rich in polyphenols, which were characterized by high-performance liquid chromatography (HPLC) separation coupled to UV/DAD and ESI-M...
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| Main Authors: | , , , , , , , , |
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| Format: | Book |
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MDPI AG,
2022-01-01T00:00:00Z.
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| Summary: | In this study, an autochthonous variety of sweet cherry (<i>Prunus avium</i> L.), namely "Moretta di Vignola", was processed to prepare extracts rich in polyphenols, which were characterized by high-performance liquid chromatography (HPLC) separation coupled to UV/DAD and ESI-MS<sup>n</sup> analysis. Then, a sweet cherry anthocyanin-rich extract (ACE) was prepared, fully characterized and tested for its activity against Parkinson's disease (PD) in cellular (BV2 microglia and SH-SY5Y neuroblastoma) and in <i>Drosophila melanogaster</i> rotenone (ROT)-induced model. The extract was also evaluated for its antioxidant activity on <i>Caenorhabditis elegans</i> by assessing nematode resistance to thermal stress. In both cell lines, ACE reduced ROT-induced cell death and it decreased, alone, cellular reactive oxygen species (ROS) content while reinstating control-like ROS values after ROT-induced ROS rise, albeit at different concentrations of both compounds. Moreover, ACE mitigated SH-SY5Y cell cytotoxicity in a non-contact co-culture assay with cell-free supernatants from ROT-treated BV-2 cells. ACE, at 50 µg/mL, ameliorated ROT (250 μM)-provoked spontaneous (24 h duration) and induced (after 3 and 7 days) locomotor activity impairment in <i>D. melanogaster</i> and it also increased survival and counteracted the decrease in fly lifespan registered after exposure to the ROT. Moreover, heads from flies treated with ACE showed a non-significant decrease in ROS levels, while those exposed to ROT markedly increased ROS levels if compared to controls. ACE + ROT significantly placed the ROS content to intermediate values between those of controls and ROT alone. Finally, ACE at 25 µg/mL produced a significant increase in the survival rate of nematodes submitted to thermal stress (35 °C, 6-8 h), at the 2nd and 9th day of adulthood. All in all, ACE from Moretta cherries can be an attractive candidate to formulate a nutraceutical product to be used for the prevention of oxidative stress-induced disorders and related neurodegenerative diseases. |
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| Item Description: | 10.3390/antiox11020211 2076-3921 |