Spread of Linezolid-Resistant <i>Enterococcus</i> spp. in Human Clinical Isolates in the Czech Republic
The aim of this study was to map and investigate linezolid resistance mechanisms in linezolid-resistant enterococci in the Czech Republic from 2009 to 2019. Altogether, 1442 isolates of <i>Enterococcus faecium</i> and <i>Enterococcus faecalis</i> were examined in the National...
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Format: | Book |
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MDPI AG,
2021-02-01T00:00:00Z.
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Summary: | The aim of this study was to map and investigate linezolid resistance mechanisms in linezolid-resistant enterococci in the Czech Republic from 2009 to 2019. Altogether, 1442 isolates of <i>Enterococcus faecium</i> and <i>Enterococcus faecalis</i> were examined in the National Reference Laboratory for Antibiotics. Among them, 8% of isolates (<i>n</i> = 115) were resistant to linezolid (<i>E. faecium</i>/<i>n</i> = 106, <i>E. faecalis</i>/<i>n</i> = 9). Only three strains of <i>E. faecium</i> were resistant to tigecycline, 72.6% of isolates were resistant to vancomycin. One isolate of <i>E. faecium</i> harbored the <i>cfr</i> gene. The majority (87%, <i>n</i> = 11) of <i>E. faecium</i> strains were resistant to linezolid because of the mutation G2576T in the domain V of the 23S rRNA. This mutation was detected also in two strains of <i>E. faecalis</i>. The presence of the <i>optrA</i> gene was the dominant mechanism of linezolid resistance in <i>E. faecalis</i> isolates. None of enterococci contained <i>cfrB</i>, <i>poxtA</i> genes, or any amino acid mutation in genes encoding ribosomal proteins. No mechanism of resistance was identified in 4 out of 106 <i>E. faecium</i> linezolid resistant isolates in this study. Seventeen sequence types (STs) including four novel STs were identified in this work. Clonal complex CC17 was found in all <i>E. faecium</i> isolates. |
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Item Description: | 10.3390/antibiotics10020219 2079-6382 |