Cytotoxic Effect of the Her-2/Her-1 Inhibitor PKI-166 on Renal Cancer Cells Expressing the Connexin 32 Gene

• Main Authors: Eriko Fujimoto (Author), Tomohiro Yano (Author), Hiromi Sato (Author), Kiyokazu Hagiwara (Author), Hiroshi Yamasaki (Author), Sumiko Shirai (Author), Keiko Fukumoto (Author), Hiromi Hagiwara (Author), Etsuko Negishi (Author), Koichi Ueno (Author)
• Format: Book
• Published: Elsevier, 2005-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

We have reported that connexin (Cx) 32 acts as a tumor suppressor gene in renal cancer cells partly due to Her-2 inactivation. Here, we determined if a Her-2/Her-1 inhibitor (PKI-166) can enhance the tumor-suppressive effect of Cx32 in Caki-2 cells from human renal cell carcinoma. The expression of Cx32 in Caki-2 cells was required for PKI-166-induced cytotoxic effect at lower doses. The cyctotoxicity was dependent on the occurrence of apoptosis and partly mediated by Cx32-driven gap junction intercellular communications. These results suggest that PKI-166 further supports the tumor-suppressive effect of the Cx32 gene in renal cancer cells through the induction of apoptosis. Keywords:: connexin 32, PKI-166, renal cancer cell

Item Description

1347-8613
10.1254/jphs.scj04009x