Programmable Molecular Scissors: Applications of a New Tool for Genome Editing in Biotech
Targeted genome editing is an advanced technique that enables precise modification of the nucleic acid sequences in a genome. Genome editing is typically performed using tools, such as molecular scissors, to cut a defined location in a specific gene. Genome editing has impacted various fields of bio...
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| Main Authors: | , , , , , , |
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| Format: | Book |
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Elsevier,
2019-03-01T00:00:00Z.
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| Online Access: | Connect to this object online. |
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| Summary: | Targeted genome editing is an advanced technique that enables precise modification of the nucleic acid sequences in a genome. Genome editing is typically performed using tools, such as molecular scissors, to cut a defined location in a specific gene. Genome editing has impacted various fields of biotechnology, such as agriculture; biopharmaceutical production; studies on the structure, regulation, and function of the genome; and the creation of transgenic organisms and cell lines. Although genome editing is used frequently, it has several limitations. Here, we provide an overview of well-studied genome-editing nucleases, including single-stranded oligodeoxynucleotides (ssODNs), transcription activator-like effector nucleases (TALENs), zinc-finger nucleases (ZFNs), and CRISPR-Cas9 RNA-guided nucleases (CRISPR-Cas9). To this end, we describe the progress toward editable nuclease-based therapies and discuss the minimization of off-target mutagenesis. Future prospects of this challenging scientific field are also discussed. Keywords: genome editing, nucleases, DSB, NHEJ, HDR, ZFNs, TALENs, CRISPR-Cas9, ssODNs, off-target mutagenesis |
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| Item Description: | 2162-2531 10.1016/j.omtn.2018.11.016 |