Resveratrol attenuates staphylococcal enterotoxin B-activated immune cell metabolism via upregulation of miR-100 and suppression of mTOR signaling pathway
Acute Respiratory Distress Syndrome (ARDS) is triggered by a variety of insults, such as bacterial and viral infections, including SARS-CoV-2, leading to high mortality. In the murine model of ARDS induced by Staphylococcal enterotoxin-B (SEB), our previous studies showed that while SEB triggered 10...
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Frontiers Media S.A.,
2023-02-01T00:00:00Z.
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|---|---|---|---|
| 001 | doaj_bc02f574a3a14b878a30c0c4586d91d8 | ||
| 042 | |a dc | ||
| 100 | 1 | 0 | |a Hasan Alghetaa |e author |
| 700 | 1 | 0 | |a Amira Mohammed |e author |
| 700 | 1 | 0 | |a Narendra Singh |e author |
| 700 | 1 | 0 | |a Kiesha Wilson |e author |
| 700 | 1 | 0 | |a Goushuai Cai |e author |
| 700 | 1 | 0 | |a Nagireddy Putluri |e author |
| 700 | 1 | 0 | |a Mitzi Nagarkatti |e author |
| 700 | 1 | 0 | |a Prakash Nagarkatti |e author |
| 245 | 0 | 0 | |a Resveratrol attenuates staphylococcal enterotoxin B-activated immune cell metabolism via upregulation of miR-100 and suppression of mTOR signaling pathway |
| 260 | |b Frontiers Media S.A., |c 2023-02-01T00:00:00Z. | ||
| 500 | |a 1663-9812 | ||
| 500 | |a 10.3389/fphar.2023.1106733 | ||
| 520 | |a Acute Respiratory Distress Syndrome (ARDS) is triggered by a variety of insults, such as bacterial and viral infections, including SARS-CoV-2, leading to high mortality. In the murine model of ARDS induced by Staphylococcal enterotoxin-B (SEB), our previous studies showed that while SEB triggered 100% mortality, treatment with Resveratrol (RES) completely prevented such mortality by attenuating inflammation in the lungs. In the current study, we investigated the metabolic profile of SEB-activated immune cells in the lungs following treatment with RES. RES-treated mice had higher expression of miR-100 in the lung mononuclear cells (MNCs), which targeted mTOR, leading to its decreased expression. Also, Single-cell RNA-seq (scRNA seq) unveiled the decreased expression of mTOR in a variety of immune cells in the lungs. There was also an increase in glycolytic and mitochondrial respiration in the cells from SEB + VEH group in comparison with SEB + RES group. Together these data suggested that RES alters the metabolic reprogramming of SEB-activated immune cells, through suppression of mTOR activation and its down- and upstream effects on energy metabolism. Also, miR-100 could serve as novel potential therapeutic molecule in the amelioration of ARDS. | ||
| 546 | |a EN | ||
| 690 | |a MiR-100 | ||
| 690 | |a ARDS (acute respiratory disease syndrome) | ||
| 690 | |a T-cell metabolism | ||
| 690 | |a mTOR | ||
| 690 | |a staphylococcal enterotoxin B (SEB) | ||
| 690 | |a resveratrol | ||
| 690 | |a Therapeutics. Pharmacology | ||
| 690 | |a RM1-950 | ||
| 655 | 7 | |a article |2 local | |
| 786 | 0 | |n Frontiers in Pharmacology, Vol 14 (2023) | |
| 787 | 0 | |n https://www.frontiersin.org/articles/10.3389/fphar.2023.1106733/full | |
| 787 | 0 | |n https://doaj.org/toc/1663-9812 | |
| 856 | 4 | 1 | |u https://doaj.org/article/bc02f574a3a14b878a30c0c4586d91d8 |z Connect to this object online. |