Polymorphism specific Allele Frequencies on Cervical Cancer
Objective: To evaluate the correlation of allele frequencies of IL-6 polymorphisms between healthy women and cervical cancer patients. Methods: The study involved gynecologists diagnosing abnormal cervical tissue in 100 women aged 17-60. Each woman provided two tissue samples: one for pap smear anal...
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| Format: | Book |
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Indonesian Society of Obstetrics and Gynecology,
2024-07-01T00:00:00Z.
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| Summary: | Objective: To evaluate the correlation of allele frequencies of IL-6 polymorphisms between healthy women and cervical cancer patients. Methods: The study involved gynecologists diagnosing abnormal cervical tissue in 100 women aged 17-60. Each woman provided two tissue samples: one for pap smear analysis and one for genetic research, along with a blood sample for IL-6 polymorphism analysis. Traditional polymerase chain reaction (PCR) was used for genetic analysis to confirm diagnoses. Allele-specific PCR (AS-PCR) was utilized to identify allelic polymorphisms. Pap smears identified cervical intraepithelial neoplasia (CIN) II and III, characterized by dysplastic cells and mitotic figures. Results: The diagnostic PCR data demonstrated that 36% of participants were HPV-infected, with the greatest infection rates (50%). The AS-PCR reported that the IL-6 (rs1800795) gene detected at 174 G/C position was presented with GG, GC, and CC genotypes. Conclusion: The result showed a significant alteration in the IL-6 (rs1800795) gene, strongly correlating with cervical cancer based on human papillomavirus infection. Keywords: cervix cancer, human papillomavirus, interleukin-6 polymorphism, genetic analysis. |
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| Item Description: | 2338-6401 2338-7335 10.32771/inajog.v12i3.2093 |