Characterization of hyperimmune anti-human serum produced in sheep

• Main Authors: Jessica Ocalia Solana Rodríguez (Author), Laura María Reyes Díaz (Author), Victoria Esther Gonzalez Ramírez (Author), Miriam San Juan Bosco Lastre González (Author), Yusnaby Borrego González (Author), Martha Leonor Paradoa Pérez (Author), Irma Vega García (Author), Raúl Ramos Pupo (Author), Oliver Pérez (Author)
• Format: Book
• Published: Universidad de Ciencias Médicas de La Habana, 2020-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

<p><strong>Introduction:</strong> Immunoelectrophoresis is a precipitation technique that allows the characterization of complex biological samples. The Immunology Department of the Institute of Basic and Pre-Clinical Sciences "Victoria de Girón" has a hyperimmune antiserum obtained by immunization of sheep against human serum total proteins and it also has an anti-human IgA antiserum obtained from human colostrum.<strong> </strong></p><p><strong>Objective</strong>: The aim of this study was to identify IgG, IgM and IgA in human serum and to determine response to anti-human IgM in human colostral IgA with antiserum obtained in sheep.</p><p><strong>Material and Methods.</strong> An observational descriptive cross-sectional study was conducted from November 2017 to June 2018. Immunoelectrophoresis of normal human serum was performed using hyperimmune antiserum.</p><p><strong>Results</strong>: These procedures allowed to identify IgG, IgM and IgA in addition to albumin and other protein fractions and to determine response to anti-human IgM in human colostral IgA with antiserum obtained in sheep.</p><strong>Conclusions: </strong>This work allowed us to identify and determine significant anti-class responses of immunoglobulins in the sample studied.

Item Description

1729-519X