Human papillomavirus in amniotic fluid

• Main Authors: Swan David C (Author), Tucker Ruth (Author), Lee Daisy R (Author), Roulston Diane (Author), Bailey Joanne M (Author), Ruffin Mack T (Author), Unger Elizabeth R (Author)
• Format: Book
• Published: BMC, 2006-09-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: 10.1186/1471-2393-6-28; 1471-2393
• Keywords: Gynecology and obstetrics; RG1-991
• Source: BMC Pregnancy and Childbirth, Vol 6, Iss 1, p 28 (2006)

<p>Abstract</p> <p>Background</p> <p>There is evidence to suggest that human papillomavirus (HPV) can cross the placenta resulting in in-utero transmission. The goal of this study was to determine if HPV can be detected in amniotic fluid from women with intact amniotic membranes.</p> <p>Methods</p> <p>Residual amniotic fluid and cultured cell pellets from amniocentesis performed for prenatal diagnosis were used. PGMY09/11 L1 consensus primers and GP5+/GP6+ primers were used in a nested polymerase chain reaction assay for HPV.</p> <p>Results</p> <p>There were 146 paired samples from 142 women representing 139 singleton pregnancies, 2 twin pregnancies, and 1 triplet pregnancy. The women were 78% Caucasian, 5% African American, 14% Asian, and 2% Hispanic. The average age was 35.2 years with a range of 23-55 years. All samples were β-globin positive. HPV was not detected in any of the paired samples.</p> <p>Conclusion</p> <p>Given the age range, race, and ethnicity of the study population, one would anticipate some evidence of HPV if it could easily cross the placenta, but there was none.</p>