Glucosyltransferase B/C expression in Streptococcus mutans of rampant and caries-free children

• Main Author: Yetty Herdiati H. Nonong (Author)
• Format: Book
• Published: Universitas Airlangga, 2009-12-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

<span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Background: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Streptococcus mutans (S. mutans) as specific bacteria causing dental caries have cariogenic characteristic related </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>to glucosyltransferase (gtf) B/C that can change sucrose into insoluble glucan. Insoluble glucan functions as an attachment media and </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>bacteria colonization, and also as a source of extracellular polysaccharide which is needed for the bacteria and may lead to caries </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>formation. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Purpose: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>The aim of this study was to find out the gtf B/C expression in isolated S. mutans from dental plaque of rampant </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>and caries-free children. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Methods: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>An observational study was done on 96 isolated bacteria grown in sucrose and bacitracin containing </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>media, which include S. mutans INA 99, S. Mutans EU3, S.mutans EU7, S.EU10a, and S.mutans 10b. PCR technique was used as </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>amplification technique for gtf B/C. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Result: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>This study showed that gtf B/C gene was found in S. mutans, S. constellatus, S. bovis, </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>S. anginosus, L. fermentum, L. salivarius, and Kleibsiella oxytoca. The presence of gtf B/C gene was found in 9 of 10 samples identified in </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>the sample of rampant caries children. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Conclusion: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>The gtf B/C enzyme was found not only in S. mutans, but also in other bacteria.</em></span></span></span></span></span></span></span></span><br style="font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: -webkit-auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px;" /></span></span></span></span></span></span></span></span></span></span>

Item Description

1978-3728
2442-9740
10.20473/j.djmkg.v42.i4.p199-203