Triggering receptor expressed on myeloid cells − 1 (Trem-1) on blood neutrophils is associated with cytokine inducibility in human <it>E</it>. <it>coli</it> sepsis

• Main Authors: van Bremen Tobias (Author), Drömann Daniel (Author), Luitjens Karin (Author), Dodt Christoph (Author), Dalhoff Klaus (Author), Goldmann Torsten (Author), Schaaf Bernhard (Author)
• Format: Book
• Published: BMC, 2013-02-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: 10.1186/1746-1596-8-24; 1746-1596
• Keywords: Triggering receptor expressed on myeloid cells − 1 (Trem-1); Sepsis; Hyporesponsiveness; Pattern recognition receptors (PRR); Neutrophils; Endotoxin tolerance; Pathology; RB1-214
• Source: Diagnostic Pathology, Vol 8, Iss 1, p 24 (2013)

<p>Abstract</p> <p>Background</p> <p>Bacterial sepsis induced immunsuppression via antigen hyporesponsibility increases the risk of nosokomial infections and mortality. Pattern recognition receptors (PRR) might have a central role in the pathophysiology of hyporesponsibility.</p> <p>Methods</p> <p>In this study we evaluated in a human <it>E</it>. <it>coli</it> sepsis cohort, the role of PRR including TLR's and Trem-1. Expression of Trem-1, TLR2, TLR4, CD14 and HLA-DR on blood monozytes and neutrophils were examined using flow cytometry from 22 patients with E. coli sepsis and 6 healthy controls. LPS and LTA stimulated TNF alpha, IL-10, IL-8 and IL-6 production was studied in a 24 h whole blood assay. Free cytokine serum concentration of TNF alpha, PCT and IP-10 were evaluated.</p> <p>Results</p> <p>We found a significant higher expression of Trem-1 and TLR-2 on monocytes and neutrophils in patients compared to healthy volunteers. TLR2 expression (p < 0.05) was higher and HLA-DR lower (p < 0.05) on monocytes of patients with severe sepsis compared to patients with simple sepsis. Trem-1 expression was tendentially higher (p = 0,07) on monocytes and lower on neutrophils of patients with severe sepsis. Trem-1 expression on neutrophils was associated with the IL-10 (LPS: r = 0,61, p < 0.02) and TNF-α inducibility (LPS: r = 0,78, p < 0,002). In addition Trem-1 expression on neutrophils shows a negative correlation to the serum levels of TNF alpha (r = −0,63; p < 0,005), IP-10 (r = −0,5; p < 0,035) and procalcitonin (r = −0,59; p < 0,007).</p> <p>Conclusions</p> <p>Patients with <it>E</it>. <it>coli</it> sepsis are characterized by an association of Trem-1 expression on blood neutrophils with cytokine inducibility. The TREM-1 pathway on neutrophils might play a role in producing an adequate inflammatory and bactericidal response in bacterial sepsis.</p> <p>Virtual Slides</p> <p>The virtual slide(s) for this article can be found here: <url>http://www.diagnosticpathology.diagnomx.eu/vs/4441869398748313</url></p>