Danlou Tablet Activates Autophagy of Vascular Adventitial Fibroblasts Through PI3K/Akt/mTOR to Protect Cells From Damage Caused by Atherosclerosis
Danlou tablet (DLT), a commercial Chinese patent medicine, has been widely used to treat cardiovascular diseases for many years. Atherosclerosis (AS) is the leading cause of cardiovascular disease. Increasing evidence indicates that autophagy plays a vital role in the development of AS. Here we inve...
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Frontiers Media S.A.,
2021-11-01T00:00:00Z.
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| LEADER | 00000 am a22000003u 4500 | ||
|---|---|---|---|
| 001 | doaj_bf6afad25e8449d7a812bff9b5448a7f | ||
| 042 | |a dc | ||
| 100 | 1 | 0 | |a Li Wang |e author |
| 700 | 1 | 0 | |a Tong Wu |e author |
| 700 | 1 | 0 | |a Chunying Si |e author |
| 700 | 1 | 0 | |a He Wang |e author |
| 700 | 1 | 0 | |a Ke Yue |e author |
| 700 | 1 | 0 | |a Shasha Shang |e author |
| 700 | 1 | 0 | |a Xiaohui Li |e author |
| 700 | 1 | 0 | |a Yushan Chen |e author |
| 700 | 1 | 0 | |a Huaimin Guan |e author |
| 245 | 0 | 0 | |a Danlou Tablet Activates Autophagy of Vascular Adventitial Fibroblasts Through PI3K/Akt/mTOR to Protect Cells From Damage Caused by Atherosclerosis |
| 260 | |b Frontiers Media S.A., |c 2021-11-01T00:00:00Z. | ||
| 500 | |a 1663-9812 | ||
| 500 | |a 10.3389/fphar.2021.730525 | ||
| 520 | |a Danlou tablet (DLT), a commercial Chinese patent medicine, has been widely used to treat cardiovascular diseases for many years. Atherosclerosis (AS) is the leading cause of cardiovascular disease. Increasing evidence indicates that autophagy plays a vital role in the development of AS. Here we investigated whether DLT could activate autophagy to improve AS and further clarified its underlying mechanisms. In an ApoE−/− mice model, the results of Oil red O, Masson's trichrome, and H&E staining techniques showed that DLT significantly inhibited lipid accumulation and fibrosis formation in atherosclerotic plaque tissue. DLT also inhibited serum triglyceride, cholesterol, and low-density lipoprotein levels and suppressed serum levels of inflammatory factors interleukin-6 and tumor necrosis factor-α in ApoE−/− mice. Moreover, DLT suppressed proliferation, migration, and invasion of human vascular adventitial fibroblasts (HVAFs) by inhibiting the PI3K/Akt/mTOR pathway. In addition, western blot analysis showed that Danlou tablet treatment decreased the expression of p62 and increased Beclin 1 and LC3 I -to-LC3 II ratios in HVAFs. The role of autophagy in treating atherosclerosis by DLT is confirmed by 3-methyladenine (autophagy inhibitor) and rapamycin (autophagy activator) in HVAFs. In summary, DLT activated PI3K/Akt/mTOR-mediated autophagy of vascular adventitial fibroblasts to protect cells from damage caused by atherosclerosis. | ||
| 546 | |a EN | ||
| 690 | |a atherosclerosis | ||
| 690 | |a danlou tablet | ||
| 690 | |a autophagy | ||
| 690 | |a PI3K/AKT/mTOR | ||
| 690 | |a Chinese patent medicine | ||
| 690 | |a Therapeutics. Pharmacology | ||
| 690 | |a RM1-950 | ||
| 655 | 7 | |a article |2 local | |
| 786 | 0 | |n Frontiers in Pharmacology, Vol 12 (2021) | |
| 787 | 0 | |n https://www.frontiersin.org/articles/10.3389/fphar.2021.730525/full | |
| 787 | 0 | |n https://doaj.org/toc/1663-9812 | |
| 856 | 4 | 1 | |u https://doaj.org/article/bf6afad25e8449d7a812bff9b5448a7f |z Connect to this object online. |