Sequence analysis of bla _CTX-M-28 , an ESBL responsible for third-generation cephalosporin resistance in <i>Enterobacteriaceae</i>, for the first time in India

• Main Authors: Kingsley Jemima (Author), Verghese Susan (Author)
• Format: Book
• Published: Wolters Kluwer Medknow Publications, 2008-04-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: 0377-4929
• Keywords: &#946;-lactamases; <i> bla</i> _CTX-M-28; <i>Enterobacteriaceae</i>; ESBL; point mutation; Pathology; RB1-214; Microbiology; QR1-502
• Source: Indian Journal of Pathology and Microbiology, Vol 51, Iss 2, Pp 218-221 (2008)

The most common group of ESBLs not belonging to the <i>bla</i> _TEM or <i>bla</i> _SHV families were termed <i>bla</i> _CTX-M , to highlight their ESBLs&#x2032; greater activity against cefotaxime than against ceftazidime. The presence of nosocomial <i>bla</i> _CTX-M-28 -producing <i>Enterobacteriaceae</i> strains has not been reported earlier in Indian hospitals. The sequences of <i>bla</i> _CTX-M-28 gene from cephalosporin-resistant <i>Enterobacteriaceae</i> were analyzed. The structural gene encodes a 290 amino-acid protein, which is most related to the <i>bla</i> _CTX-M &#946;-lactamases. The conserved K-T-G was identified in the <i>bla</i> _CTX-M-28 protein sequence, but significantly, two point mutations (N&#8594;T) and (F&#8594;S) were identified in the Y-G-N- and S-T-F-K-conserved motifs respectively. These point mutations were seen in all the three sequenced isolates