Simultaneous determination of eight major bioactive compounds in Dachengqi Tang (DT) by high-performance liquid chromatography

• Main Authors: Chen Guanyuan (Author), Zhu Zhengyan (Author), Wan Meihua (Author), Tang Wenfu (Author), Huang Xi (Author)
• Format: Book
• Published: BMC, 2008-04-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

<p>Abstract</p> <p>Background</p> <p><it>Dachengqi Tang </it>(DT) is a common traditional Chinese medicine formula for expelling <it>neire </it>('internal heat') in the stomach and intestines. There was no reliable analytical method available for the quality control of DT.</p> <p>Methods</p> <p>A high-performance liquid chromatography (HPLC) method with a reverse phase C₁₈column (150 × 4.6 mm) was developed. The mobile phase was methanol with 0.2% acetic acid. Eight markers including naringin, hesperidin, aloe emodin, rhein, honokiol, magnolol, emodin and chrysophanol were determined.</p> <p>Results</p> <p>Regression analysis revealed a linear relationship between the concentrations of the markers and the peak area ratio of the standards and internal standard. The limit of detection (S/N = 3) and the limit of qualification (RSD < 20%) ranged from 0.21 to 0.43 ng/μl and 0.76 to 1.74 ng/μl respectively. The recovery was between 95.6% and 103.4%. The tests on the samples from three batches of DT showed that the profiles of the markers did not vary significantly among batches.</p> <p>Conclusion</p> <p>A reliable HPLC method for simultaneous determination of the eight markers in DT was developed.</p>

Item Description

10.1186/1749-8546-3-5
1749-8546