Easy and rapid method for the determination of gene expression in cumulus cells incubated for oocyte maturation

<p>Abstract</p> <p>Background</p> <p>The objectives of this study were to develop an easy and rapid method for measuring gene expression in a small number of cells by real-time PCR without RNA extraction and purification, and to use this method to determine more precise...

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Bibliographic Details
Main Authors: Yamashiro Hideaki (Author), Wang Haifeng (Author), Kumamoto Kanako (Author), Terada Takato (Author)
Format: Book
Published: BMC, 2005-10-01T00:00:00Z.
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