Analysis of Ki-67 expression in oral premalignant lesions and normal oral mucosa: An immunohistochemical study

• Main Authors: Basheer H Beevi (Author), Suhas Ramananda Nayak (Author), Celestina D Peter (Author), Ajay K Haridas (Author), Lin Jacob (Author), Asaf Aboobakker (Author)
• Format: Book
• Published: Wolters Kluwer Medknow Publications, 2019-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: 0975-7406; 10.4103/JPBS.JPBS_305_18
• Keywords: Ki-67; leukoplakia; normal mucosa; oral lichen planus; Pharmacy and materia medica; RS1-441; Analytical chemistry; QD71-142
• Source: Journal of Pharmacy and Bioallied Sciences, Vol 11, Iss 6, Pp 232-235 (2019)

Aim: This study aimed to evaluate the Ki-67 expression in oral premalignant lesions and normal oral mucosa. Materials and Methods: The cases were selected on the basis of the details obtained from the patients. A total of 45 specimens were divided into three groups: Group 1 (normal mucosa), Group 2 (clinically and histologically diagnosed as oral lichen planus), and Group 3 (clinically and histologically diagnosed as leukoplakia). Specimens were prepared and the slides for Ki-67 were observed under light microscope with a magnification of ×400. The tissue samples were thoroughly examined, and the pattern of expression was analyzed semiquantitatively by counting the number of positive cells. Results: The mean positive cell count of normal mucosa was 23.20±2.89, of oral lichen planus was 42.82±2.65, and of leukoplakia was 82.14±3.10. There was a statistically significant difference of expression observed between the groups (P < 0.001). On multiple comparisons using Tukey post hoc test, a statistical difference was found between all the three groups. Conclusion: Ki-67 is an easily applicable marker of cell proliferation whose expression correlates well with the disease progression.