Magnetic Nanoparticles with On-Site Azide and Alkyne Functionalized Polymer Coating in a Single Step through a Solvothermal Process

• Main Authors: Romualdo Mora-Cabello (Author), David Fuentes-Ríos (Author), Lidia Gago (Author), Laura Cabeza (Author), Ana Moscoso (Author), Consolación Melguizo (Author), José Prados (Author), Francisco Sarabia (Author), Juan Manuel López-Romero (Author)
• Format: Book
• Published: MDPI AG, 2024-09-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: 10.3390/pharmaceutics16091226; 1999-4923
• Keywords: CBD-derivatives; magnetite; metalloorganic nanoparticles; surface functionalization; hyperthermia; SPION; Pharmacy and materia medica; RS1-441
• Source: Pharmaceutics, Vol 16, Iss 9, p 1226 (2024)

<b>Background/Objectives</b>: Magnetic Fe₃O₄ nanoparticles (MNPs) are becoming more important every day. We prepared MNPs in a simple one-step reaction by following the solvothermal method, assisted by azide and alkyne functionalized polyethylene glycol (PEG400) polymers, as well as by PEG6000 and the polyol β-cyclodextrin (βCD), which played a crucial role as electrostatic stabilizers, providing polymeric/polyol coatings around the magnetic cores. <b>Methods</b>: The composition, morphology, and magnetic properties of the nanospheres were analyzed using Transmission Electron and Atomic Force Microscopies (TEM, AFM), Nuclear Magnetic Resonance (NMR), X-ray Diffraction Diffractometry (XRD), Fourier-Transform Infrared Spectroscopy (FT-IR), Matrix-Assisted Laser Desorption/Ionization (MALDI) and Vibrating Sample Magnetometry (VSM). <b>Results</b>: The obtained nanoparticles (@Fe₃O₄-PEGs and @Fe₃O₄-βCD) showed diameters between 90 and 250 nm, depending on the polymer used and the Fe₃O₄·6H₂O precursor concentration, typically, 0.13 M at 200 °C and 24 h of reaction. MNPs exhibited superparamagnetism with high saturation mass magnetization at room temperature, reaching values of 59.9 emu/g (@Fe₃O₄-PEG6000), and no ferromagnetism. Likewise, they showed temperature elevation after applying an alternating magnetic field (AMF), obtaining Specific Absorption Rate (SAR) values of up to 51.87 ± 2.23 W/g for @Fe₃O₄-PEG6000. Additionally, the formed systems are susceptible to click chemistry, as was demonstrated in the case of the cannabidiol-propargyl derivative (CBD-Pro), which was synthesized and covalently attached to the azide functionalized surface of @Fe₃O₄-PEG400-N₃. Prepared MNPs are highly dispersible in water, PBS, and citrate buffer, remaining in suspension for over 2 weeks, and non-toxic in the T84 human colon cancer cell line, <b>Conclusions</b>: indicating that they are ideal candidates for biomedical applications.