Expression of lipocortins in human bronchial epithelial cells: effects of IL-1β , TNF-α, LPS and dexamethasone

• Main Authors: M. M. Verheggen (Author), H. I. M. de Bont (Author), P. W. C. Adriaansen-Soeting (Author), B. J. A. Goense (Author), C. J. A. M. Tak (Author), H. C. Hoogsteden (Author), P. Th. W. van Hal (Author), M. A. Versnel (Author)
• Format: Book
• Published: Hindawi Limited, 1996-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

In this study, we investigated the expression of lipocortin I and II (annexin I and I in the human bronchial epithelium, both in vivo and in vitro. A clear expression of lipocortin I and II protein was found in the epithelium in sections of bronchial tissue. In cultured human bronchial epithelial cells we demonstrated the expression of lipocortin I and II mRNA and protein using Northern blotting, FACScan analysis and ELISA. No induction of lipocortin I or II mRNA or protein was observed after incubation with dexamethasone. Stimulation of bronchial epithelial cells with IL-1β, TNF-α or LPS for 24 h did not affect the lipocortin I or II mRNA or protein expression, although PGE2 and 6-keto-PGF1α production was significantly increased. This IL-1β- and LPS-mediated increase in eicosanoids could be reduced by dexamethasone, but was not accompanied by an increase in lipocortin I or II expression. In human bronchial epithelial cells this particular glucocorticoid action is not mediated through lipocortin I or II induction.

Item Description

0962-9351
1466-1861
10.1155/S0962935196000300