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Detection and Characterization of Carbapenemase-Producing <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> from Hospital Effluents of Ouagadougou, Burkina Faso

Hospital wastewater is a recognized reservoir for resistant Gram-negative bacteria. This study aimed to screen for carbapenemase-producing <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> and their resistance determinants in two hospital effluents of Ouagadougou. Carb...

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Main Authors: Alix Bénédicte Kagambèga (Author), René Dembélé (Author), Léa Bientz (Author), Fatima M'Zali (Author), Laure Mayonnove (Author), Alassane Halawen Mohamed (Author), Hiliassa Coulibaly (Author), Nicolas Barro (Author), Véronique Dubois (Author)
Format: Book
Published: MDPI AG, 2023-09-01T00:00:00Z.
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Summary:Hospital wastewater is a recognized reservoir for resistant Gram-negative bacteria. This study aimed to screen for carbapenemase-producing <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> and their resistance determinants in two hospital effluents of Ouagadougou. Carbapenem-resistant <i>E. coli</i> and <i>K. pneumoniae</i> were selectively isolated from wastewater collected from two public hospitals in Ouagadougou, Burkina Faso. Bacterial species were identified via MALDI-TOF mass spectrometry. Carbapenemase production was studied phenotypically using antibiotic susceptibility testing via the disk diffusion method. The presence of carbapenemases was further characterized by PCR. A total of 14 <i>E</i>. <i>coli</i> (13.59%) and 19 <i>K</i>. <i>pneumoniae</i> (17.92%) carbapenemase-producing isolates were identified with different distributions. They were, respectively, <i>bla</i><sub>NDM</sub> (71.43%), <i>bla</i><sub>VIM</sub> (42.86%), <i>bla</i><sub>IMP</sub> (28.57%), <i>bla</i><sub>KPC</sub> (14.29%), <i>bla</i><sub>OXA-48</sub> (14.29%); and <i>bla</i><sub>KPC</sub> (68.42%), <i>bla</i><sub>NDM</sub> (68.42%), <i>bla</i><sub>IMP</sub> (10.53%), <i>bla</i><sub>VIM</sub> (10.53%), and <i>bla</i><sub>OXA-48</sub> (5.26%). In addition, eight (57.14%) <i>E</i>. <i>coli</i> and eleven (57.89%) <i>K</i>. <i>pneumoniae</i> isolates exhibited more than one carbapenemase, KPC and NDM being the most prevalent combination. Our results highlight the presence of clinically relevant carbapenemase-producing isolates in hospital effluents, suggesting their presence also in hospitals. Their spread into the environment via hospital effluents calls for intensive antimicrobial resistance (AMR) surveillance.
Item Description:10.3390/antibiotics12101494
2079-6382

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