Aqueous extract of Tamarindus indica fruit pulp exhibits antihyperglycaemic activity
<strong>Objective: </strong><em>Tamarindus indica </em>Linn.(<em>T.indica</em>) is a well-known plant used in traditional medicine. The plant is popular for its antidiabetic activity. However, effect so f its aqueous fruit pulp extract on carbohydrate hydrolyzing...
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Mashhad University of Medical Sciences,
2020-09-01T00:00:00Z.
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| Summary: | <strong>Objective: </strong><em>Tamarindus indica </em>Linn.(<em>T.indica</em>) is a well-known plant used in traditional medicine. The plant is popular for its antidiabetic activity. However, effect so f its aqueous fruit pulp extract on carbohydrate hydrolyzing enzymes and its glucose uptake potential were not explored.<br /> <strong>Materials and Methods: </strong>The antidiabetic activity was assessed by <em>in-vitro </em>α-amylase and α-glucosidase inhibitory assays after preliminary phytochemical analysis. MTT assay was carried out to find cytotoxicity. Glucose uptake activity of the extract was carried out using L6 myotubes.<br /> <strong>Results:</strong>The results showed a strong α-amylase inhibitory activity for the fruit pulp extract of <em>T.indica</em> compared to standard acarbose; the IC<sub>50</sub> of the fruit pulp extract of <em>T.indica</em> and acarbose was 34.19 µg/ml 34.83µM. The extract also showed moderate α-glucosidase inhibitory activity. IC<sub>50</sub> of the fruit pulp extract of <em>T.indica</em> and acarbose were 56.91µg/ml and 45.69µM respectively. The cytotoxicity assay showed IC<sub>50</sub> of >300µg/ml and ≥1000µM for the fruit pulp extract of <em>T.indica</em> and metformin. The extract showed 63.99±0.08% glucose uptake in L6 myotubes whereas metformin and insulin at 10µg/ml and 10µM exhibited an uptake of 76.99±0.3% and 84.48±0.45% glucose, respectively.<br /> <strong>Conclusion: </strong>The study revealed that the fruit pulp extract of <em>T.indica </em>Linn does not show any cytotoxic effect and has very good α-amylase and good α-glucosidase inhibitory activities. The glucose uptake potential proves its postprandial hypoglycemic effect. Hence, it may be considered an antidiabetic agent for control of postprandial hyperglycemia. |
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| Item Description: | 2228-7930 2228-7949 10.22038/ajp.2020.14787 |