Effect of Peptides on the Synthesis, Properties and Wound Healing Capacity of Silver Nanoparticles

The aim of this study is the synthesis of novel peptide-silver nanoparticle conjugates with enhanced wound healing capacity. Peptide-silver nanoparticle conjugates were synthesized using myristoyl tetrapeptide 6 (MT6) or copper tripeptide 1 (CuTP1). Peptide-free silver nanoparticles (AgNP) were synt...

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Main Authors: Afroditi Papaioannou (Author), Angeliki Liakopoulou (Author), Dimitris Papoulis (Author), Eleni Gianni (Author), Patroula Gkolfi (Author), Eleni Zygouri (Author), Sophia Letsiou (Author), Sophia Hatziantoniou (Author)
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Published: MDPI AG, 2023-10-01T00:00:00Z.
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042 |a dc 
100 1 0 |a Afroditi Papaioannou  |e author 
700 1 0 |a Angeliki Liakopoulou  |e author 
700 1 0 |a Dimitris Papoulis  |e author 
700 1 0 |a Eleni Gianni  |e author 
700 1 0 |a Patroula Gkolfi  |e author 
700 1 0 |a Eleni Zygouri  |e author 
700 1 0 |a Sophia Letsiou  |e author 
700 1 0 |a Sophia Hatziantoniou  |e author 
245 0 0 |a Effect of Peptides on the Synthesis, Properties and Wound Healing Capacity of Silver Nanoparticles 
260 |b MDPI AG,   |c 2023-10-01T00:00:00Z. 
500 |a 10.3390/pharmaceutics15102471 
500 |a 1999-4923 
520 |a The aim of this study is the synthesis of novel peptide-silver nanoparticle conjugates with enhanced wound healing capacity. Peptide-silver nanoparticle conjugates were synthesized using myristoyl tetrapeptide 6 (MT6) or copper tripeptide 1 (CuTP1). Peptide-free silver nanoparticles (AgNP) were synthesized using NaBH4 and sodium citrate and were used as control. The addition of the peptides during or after the synthesis of nanoparticles and its impact on the properties of the synthesized peptide-silver nanoparticle conjugates were assessed. The monitoring of the synthesis of nanoparticles was achieved using ultraviolet-visible spectrophotometry (UV-/Vis). The characteristics and colloidal stability of the nanoparticles (size and ζ-potential distribution, morphology, composition and structure) were monitored using dynamic laser scattering (DLS), transmission electron microscopy (TEM), atomic absorption spectroscopy (AAS) and X-ray diffraction (XRD). The wound healing capacity of the peptide-silver nanoparticle conjugates was assessed using scratch test assay on fibroblasts (NIH/3T3). The results indicated that the addition of the peptides during the synthesis of nanoparticles lead to better yield of the reaction and more effective capping while the size distribution and ζ-potential of the conjugates indicated long-term colloidal stability. The MT6-AgNP conjugate exhibited 71.97 ± 4.35% wound closure, which was about 5.48-fold higher (<i>p</i> < 0.05) than the corresponding free MT6. The CuTP1-AgNP conjugate exhibited 62.37 ± 18.33% wound closure that was better by 2.82 fold (<i>p</i> < 0.05) compared to the corresponding free CuTP1. Both peptides led to the synthesis of silver nanoparticle conjugates with enhanced wound healing capacity compared to the respective free peptide or to the peptide-free AgNP (29.53 ± 4.71% wound closure, <i>p</i> < 0.05). Our findings demonstrated that the synthetized peptide-silver nanoparticle conjugates are promising ingredients for wound care formulation. 
546 |a EN 
690 |a AgNP 
690 |a silver nanoparticles 
690 |a peptide conjugates 
690 |a wound healing 
690 |a scratch test 
690 |a myristoyl tetrapeptide 6 
690 |a Pharmacy and materia medica 
690 |a RS1-441 
655 7 |a article  |2 local 
786 0 |n Pharmaceutics, Vol 15, Iss 10, p 2471 (2023) 
787 0 |n https://www.mdpi.com/1999-4923/15/10/2471 
787 0 |n https://doaj.org/toc/1999-4923 
856 4 1 |u https://doaj.org/article/c2023e3696e54363bc20b82792e2d2db  |z Connect to this object online.