Lyophilized Symbiotic Mitigates Mucositis Induced by 5-Fluorouracil

• Main Authors: Bruna Savassi (Author), Bárbara F. Cordeiro (Author), Sara H. Silva (Author), Emiliano R. Oliveira (Author), Giovanna Belo (Author), Alessandra Gomes Figueiroa (Author), Maria Izabel Alves Queiroz (Author), Ana Maria Caetano Faria (Author), Juliana Alves (Author), Tales Fernando da Silva (Author), Gabriela Munis Campos (Author), Erick A. Esmerino (Author), Ramon S. Rocha (Author), Monica Q. Freitas (Author), Marcia C. Silva (Author), Adriano G. Cruz (Author), Kátia Duarte Vital (Author), Simone O.A. Fernandes (Author), Valbert N. Cardoso (Author), Leonardo Borges Acurcio (Author), Gwénaël Jan (Author), Yves Le Loir (Author), Alfonso Gala-Garcia (Author), Fillipe Luiz R. do Carmo (Author), Vasco Azevedo (Author)
• Format: Book
• Published: Frontiers Media S.A., 2021-12-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

Mucositis is an adverse effect of cancer chemotherapies using 5-Fluorouracil (5-FU). It is characterized by mucosal inflammation, pain, diarrhea, and weight loss. Some studies reported promising healing effects of probiotic strains, when associated with prebiotics, as adjuvant treatment of mucositis. We developed a lyophilized symbiotic product, containing skimmed milk, supplemented with whey protein isolate (WPI) and with fructooligosaccharides (FOS), and fermented by Lactobacillus casei BL23, Lactiplantibacillus plantarum B7, and Lacticaseibacillus rhamnosus B1. In a mice 5-FU mucositis model, this symbiotic lyophilized formulation was able to reduce weight loss and intestinal permeability. This last was determined in vivo by quantifying blood radioactivity after oral administration of 99mTc-DTPA. Finally, histological damages caused by 5-FU-induced mucositis were monitored. Consumption of the symbiotic formulation caused a reduced score of inflammation in the duodenum, ileum, and colon. In addition, it decreased levels of pro-inflammatory cytokines IL-1β, IL-6, IL-17, and TNF-α in the mice ileum. The symbiotic product developed in this work thus represents a promising adjuvant treatment of mucositis.

Item Description

1663-9812
10.3389/fphar.2021.755871