Two-Dimensional and Spheroid-Based Three-Dimensional Cell Culture Systems: Implications for Drug Discovery in Cancer

• Main Authors: Anali del Milagro Bernabe Garnique (Author), Natália Sudan Parducci (Author), Lívia Bassani Lins de Miranda (Author), Bruna Oliveira de Almeida (Author), Leonardo Sanches (Author), João Agostinho Machado-Neto (Author)
• Format: Book
• Published: MDPI AG, 2024-06-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

The monolayer (two-dimensional or 2D) cell culture, while widely used, lacks fidelity in replicating vital cell interactions seen in vivo, leading to a shift toward three-dimensional (3D) models. Although monolayers offer simplicity and cost-effectiveness, spheroids mimic cellular environments bette...