Expression of Group I Metabotropic Glutamate Receptors in Rat Hippocampal Cells in Culture and Their Characterization by Intracellular Calcium Ion Dynamics

• Main Authors: Yoshitoku Yoshida (Author), Nagisa Matsumoto (Author), Remi Tsuchiya (Author), Mitsuhiro Morita (Author), Hiroyoshi Miyakawa (Author), Yoshihisa Kudo (Author)
• Format: Book
• Published: Elsevier, 2003-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

The distribution of group I metabotropic glutamate receptors in rat hippocampal cells in culture was examined by calcium imaging and immunocytochemistry. To distinguish different cell types in the culture, the effects of t-ACPD ((1S,3R)-1-aminocyclopentane-1,3-dicarboxylic acid) and of NMDA (N-methyl-D-aspartate) were examined. About 40% of the cultured cells showed either a transient increase or a sustained or oscillatory increase in the intracellular calcium concentration ([Ca2+]i) during t-ACPD administration, while about 60% of the cells showed a sustained [Ca2+]i increase in response to NMDA. Cells that showed an oscillatory [Ca2+]i change during t-ACPD administration did not respond to NMDA administration, while cells that showed a sustained [Ca2+]i increase during NMDA administration did not show any oscillatory response to t-ACPD. After pharmacological examination using those two agonists, the cultured cells were subjected to immunocytochemistry using anti-GFAP and ant-MAP-2 antibodies to distinguish, respectively, astrocytes and neurons. All cells responding to NMDA with a sustained [Ca2+]i increase were MAP-2-positive, whereas all cells showing either oscillatory or sustained [Ca2+]i increase in response to t-ACPD were GFAP-positive. The present results show that, in these cultures, group I metabotropic glutamate receptors are mainly expressed on glial cells and contribute to dynamic [Ca2+]i changes in astrocytes.

Item Description

1347-8613
10.1254/S1347-8613(19)32656-8