Overexpression of Metastatic Related MicroRNAs, Mir-335 and Mir-10b, by Staphylococcal Enterotoxin B in the Metastatic Breast Cancer Cell Line

• Main Authors: Mohammad Foad Heidary (Author), Hamideh Mahmoodzadeh Hosseini (Author), Elnaz Mehdizadeh Aghdam (Author), Mohammad Reza Nourani (Author), Reza Ranjbar (Author), Reza Mirnejad (Author), Abbas Ali Imani Fooladi (Author)
• Format: Book
• Published: Tabriz University of Medical Sciences, 2015-06-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

Purpose : One of the advanced cancer therapy strategies is immune - stimulating compound based immunotherapy Staphylococcal enterotoxin B (SEB) is one of the potent superantigens, which can efficiently activate antitumor immune response to eradicate tumor growth and inhibit metastasis. Herein, we evaluated the effect of SEB on the expression of two master microRN As, mir - 335 and mir - 10b, involved in metastasis. Methods : A metastatic breast cancer cell line MDA - MB231was treated with four different concentrations of SEB, including 10, 10 2 , 10 3 and 10 4 ng/ml, for 24 and 48 hours. To identify the cytotoxic effect of S EB, treated cells were examined by MTT assay. The stem loop RT - PCR (TaqMan) was used to analyze the mir - 335 and mir - 10b expression. Results : Results showed that SEB significantly increased the expression of mir - 335 both after 24 and 48 hours ( p v < 0.001 and p v < 0.05, respectively). No significant differences were found in the mir - 10b expression. Conclusion : Moreover, our findings demonstrated no cytotoxic effect of SEB on the treated cells. Our results suggest that SEB probably induces its anti - m etastatic effect via the expression regulation of the main genes which contributes to metastasis.

Item Description

10.15171/apb.2015.035
2228-5881
2251-7308