Honeybee Royal Jelly and Nobiletin Stimulate CRE-Mediated Transcription in ERK-Independent and -Dependent Fashions, Respectively, in PC12D Cells

• Main Authors: Hironori Fujiwara (Author), Ako Kogure (Author), Masahiro Sakamoto (Author), Tohru Yamakuni (Author), Yoshihiro Mimaki (Author), Kiyoshi Murata (Author), Nobuyuki Hitomi (Author), Kikuji Yamaguchi (Author), Yasushi Ohizumi (Author)
• Format: Book
• Published: Elsevier, 2011-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

Abstract.: To prove the pharmacological actions of honeybee royal jelly (RJ) on the nervous system, we examined the effects of RJ on CRE-mediated transcription. RJ increased CRE-mediated transcription in PC12D cells. Moreover, CRE-mediated transcriptional activity by RJ was enhanced by nobiletin. U0126, a MEK inhibitor, inhibited CRE-mediated transcription by combining RJ plus nobiletin without affecting transcription by RJ alone. These results suggest that RJ stimulates CRE-mediated transcription via an ERK-independent cascade, whereas the increasing CRE-mediated transcriptional effect by nobiletin is dependent on ERK phosphorylation. Combining RJ plus nobiletin may activate effectively neuronal functions via enhancement of CRE-mediated transcription. Keywords:: honeybee royal jelly, CRE-mediated transcription, Alzheimer's disease

Item Description

1347-8613
10.1254/jphs.11001SC