Longitudinal proteomic profiling of the inflammatory response in dengue patients.

• Główni autorzy: Fadel Muhammad Garishah (Autor), Collins K Boahen (Autor), Nadira Vadaq (Autor), Setyo G Pramudo (Autor), Rahajeng N Tunjungputri (Autor), Silvita Fitri Riswari (Autor), Ronald P van Rij (Autor), Bachti Alisjahbana (Autor), Muhammad Hussein Gasem (Autor), André J A M van der Ven (Autor), Quirijn de Mast (Autor)
• Format: Książka
• Wydane: Public Library of Science (PLoS), 2023-01-01T00:00:00Z.
• Hasła przedmiotowe: article
• Dostęp online: Connect to this object online.

Streszczenie

<h4>Background</h4>The immunopathogenesis of dengue virus (DENV) infection remains incompletely understood. To increase our understanding of inflammatory response in non-severe dengue, we assessed longitudinal changes in the inflammatory proteome in patients with an acute DENV infection.<h4>Methods</h4>Using a multiplex proximity extension assay (PEA), we measured relative levels of 368 inflammatory markers in plasma samples from hospitalized patients with non-severe DENV infection in the acute (n = 43) and convalescence (n = 35) phase of the infection and samples of healthy controls (n = 10).<h4>Results</h4>We identified 203 upregulated and 39 downregulated proteins in acute versus convalescent plasma samples. The upregulated proteins had a strong representation of interferon (IFN) and IFN-inducible effector proteins, cytokines (e.g. IL-10, IL-33) and cytokine receptors, chemokines, pro-apoptotic proteins (e.g. granzymes) and endothelial markers. A number of differentially expressed proteins (DEPs) have not been reported in previous studies. Functional network analysis highlighted a central role for IFNγ, IL-10, IL-33 and chemokines. We identified different novel associations between inflammatory proteins and circulating concentrations of the endothelial glycocalyx disruption surrogate marker syndecan-1. Conclusion: This unbiased proteome analysis provides a comprehensive insight in the inflammatory response in DENV infection and its association with glycocalyx disruption.

Deskrypcja

1935-2727
1935-2735
10.1371/journal.pntd.0011041