In Vitro Study of Licorice on IL-1β-Induced Chondrocytes and In Silico Approach for Osteoarthritis
Osteoarthritis (OA) is a common degenerative joint disorder that affects joint function, mobility, and pain. The release of proinflammatory cytokines stimulates matrix metalloproteinases (MMPs) and aggrecanase production which further induces articular cartilage degradation. Hypertrophy-like changes...
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2021-12-01T00:00:00Z.
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| 001 | doaj_c46d59e1246f42b1a96ed032b346be9c | ||
| 042 | |a dc | ||
| 100 | 1 | 0 | |a Akhtar Ali |e author |
| 700 | 1 | 0 | |a YoungJoon Park |e author |
| 700 | 1 | 0 | |a Jeonghoon Lee |e author |
| 700 | 1 | 0 | |a Hyo-Jin An |e author |
| 700 | 1 | 0 | |a Jong-Sik Jin |e author |
| 700 | 1 | 0 | |a Jong-Hyun Lee |e author |
| 700 | 1 | 0 | |a Jaeki Chang |e author |
| 700 | 1 | 0 | |a Dong-Keun Kim |e author |
| 700 | 1 | 0 | |a Bonhyuk Goo |e author |
| 700 | 1 | 0 | |a Yeon Cheol Park |e author |
| 700 | 1 | 0 | |a Kang-Hyun Leem |e author |
| 700 | 1 | 0 | |a Shin Seong |e author |
| 700 | 1 | 0 | |a Wonnam Kim |e author |
| 245 | 0 | 0 | |a In Vitro Study of Licorice on IL-1β-Induced Chondrocytes and In Silico Approach for Osteoarthritis |
| 260 | |b MDPI AG, |c 2021-12-01T00:00:00Z. | ||
| 500 | |a 10.3390/ph14121337 | ||
| 500 | |a 1424-8247 | ||
| 520 | |a Osteoarthritis (OA) is a common degenerative joint disorder that affects joint function, mobility, and pain. The release of proinflammatory cytokines stimulates matrix metalloproteinases (MMPs) and aggrecanase production which further induces articular cartilage degradation. Hypertrophy-like changes in chondrocytes are considered to be an important feature of OA pathogenesis. A <i>Glycyrrhiza</i> new variety, Wongam (WG), was developed by the Korea Rural Development Administration to enhance the cultivation and quality of Glycyrrhizae Radix et Rhizoma (licorice). This study examined the regulatory effect of WG against hypertrophy-like changes such as RUNX2, Collagen X, VEGFA, MMP-13 induction, and Collagen II reduction induced by IL-1β in SW1353 human chondrocytes. Additionally, in silico methods were performed to identify active compounds in licorice to target chondrocyte hypertrophy-related proteins. WG showed inhibitory effects against IL-1β-induced chondrocyte hypertrophy by regulating both HDAC4 activation via the PTH1R/PKA/PP2A pathway and the SOX9/β-catenin signaling pathway. In silico analysis demonstrated that 21 active compounds from licorice have binding potential with 11 targets related to chondrocyte hypertrophy. Further molecular docking analysis and in vivo studies elicited four compounds. Based on HPLC, isoliquiritigenin and its precursors were identified and quantified. Taken together, WG is a potential therapeutic agent for chondrocyte hypertrophy-like changes in OA. | ||
| 546 | |a EN | ||
| 690 | |a osteoarthritis | ||
| 690 | |a chondrocyte hypertrophy | ||
| 690 | |a licorice | ||
| 690 | |a Wongam | ||
| 690 | |a Medicine | ||
| 690 | |a R | ||
| 690 | |a Pharmacy and materia medica | ||
| 690 | |a RS1-441 | ||
| 655 | 7 | |a article |2 local | |
| 786 | 0 | |n Pharmaceuticals, Vol 14, Iss 12, p 1337 (2021) | |
| 787 | 0 | |n https://www.mdpi.com/1424-8247/14/12/1337 | |
| 787 | 0 | |n https://doaj.org/toc/1424-8247 | |
| 856 | 4 | 1 | |u https://doaj.org/article/c46d59e1246f42b1a96ed032b346be9c |z Connect to this object online. |