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High Fecal Prevalence of <i>mcr</i>-Positive <i>Escherichia coli</i> in Veal Calves at Slaughter in France

The aim of this study was to determine the percentage of healthy veal calves carrying <i>mcr</i>-positive <i>E. coli</i> strains at the time of slaughter in France. Fecal samples were selectively screened for <i>mcr</i>-positive <i>E. coli</i> isolates...

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Bibliographic Details
Main Authors:	Maryse Michèle Um (Author), Véronique Dupouy (Author), Nathalie Arpaillange (Author), Clémence Bièche-Terrier (Author), Frédéric Auvray (Author), Eric Oswald (Author), Hubert Brugère (Author), Delphine Bibbal (Author)
Format:	Book
Published:	MDPI AG, 2022-08-01T00:00:00Z.
Subjects:	article
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Summary:	The aim of this study was to determine the percentage of healthy veal calves carrying <i>mcr</i>-positive <i>E. coli</i> strains at the time of slaughter in France. Fecal samples were selectively screened for <i>mcr</i>-positive <i>E. coli</i> isolates using media supplemented with colistin. Screening for <i>mcr</i> genes was also carried out in <i>E. coli</i> isolates resistant to critically important antimicrobials used in human medicine recovered from the same fecal samples. Overall, 28 (16.5%) out of the 170 veal calves tested carried <i>mcr</i>-positive <i>E. coli</i>. As some calves carried several non-redundant <i>mcr</i>-positive strains, 41 <i>mcr</i>-positive <i>E. coli</i> were recovered. Thirty-one and seven strains were positive for <i>mcr-1</i> and <i>mcr-3</i> genes, respectively, while no strain was positive for the <i>mcr-2</i> gene. Co-carriage of <i>mcr-1</i> and <i>mcr-3</i> was identified in three strains. All <i>mcr</i>-positive <i>E. coli</i> isolates, except one, were multidrug-resistant, with 56.1% being ciprofloxacin-resistant and 31.7% harboring <i>bla</i><sub>CTX-M</sub> genes. All <i>mcr-3</i>-positive <i>E. coli</i> carried <i>bla</i><sub>CTX-M</sub> genes, mainly <i>bla</i><sub>CTX-M-55</sub>. This study highlights the high prevalence of <i>mcr</i>-positive <i>E. coli</i> strains in feces of veal calves at the time of slaughter. It also points out the multidrug (including ciprofloxacin) resistance of such strains and the co-occurrence of <i>mcr-3</i> genes with <i>bla</i><sub>CTX-M-55</sub> genes.
Item Description:	10.3390/antibiotics11081071 2079-6382