Steroidogenic and maturation-inducing potency of native gonadotropic hormones in female chub mackerel, <it>Scomber japonicus</it>
<p>Abstract</p> <p>Background</p> <p>The gonadotropins (GtHs), follicle-stimulating hormone (FSH) and luteinizing hormone (LH) are produced in the pituitary gland and regulates gametogenesis through production of gonadal steroids. However, respective roles of two GtHs i...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Book |
| Published: |
BMC,
2012-09-01T00:00:00Z.
|
| Subjects: | |
| Online Access: | Connect to this object online. |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
MARC
| LEADER | 00000 am a22000003u 4500 | ||
|---|---|---|---|
| 001 | doaj_c58e0d77c9b04943a23fb18f1addf393 | ||
| 042 | |a dc | ||
| 100 | 1 | 0 | |a Ohga Hirofumi |e author |
| 700 | 1 | 0 | |a Kaneko Kensuke |e author |
| 700 | 1 | 0 | |a Shimizu Akio |e author |
| 700 | 1 | 0 | |a Kitano Hajime |e author |
| 700 | 1 | 0 | |a Selvaraj Sethu |e author |
| 700 | 1 | 0 | |a Nyuji Mitsuo |e author |
| 700 | 1 | 0 | |a Adachi Hayato |e author |
| 700 | 1 | 0 | |a Yamaguchi Akihiko |e author |
| 700 | 1 | 0 | |a Matsuyama Michiya |e author |
| 245 | 0 | 0 | |a Steroidogenic and maturation-inducing potency of native gonadotropic hormones in female chub mackerel, <it>Scomber japonicus</it> |
| 260 | |b BMC, |c 2012-09-01T00:00:00Z. | ||
| 500 | |a 10.1186/1477-7827-10-71 | ||
| 500 | |a 1477-7827 | ||
| 520 | |a <p>Abstract</p> <p>Background</p> <p>The gonadotropins (GtHs), follicle-stimulating hormone (FSH) and luteinizing hormone (LH) are produced in the pituitary gland and regulates gametogenesis through production of gonadal steroids. However, respective roles of two GtHs in the teleosts are still incompletely characterized due to technical difficulties in the purification of native GtHs.</p> <p>Methods</p> <p>Native FSH and LH were purified from the pituitaries of adult chub mackerel, <it>Scomber japonicus</it> by anion-exchange chromatography and immunoblotting using specific antisera. The steroidogenic potency of the intact chub mackerel FSH (cmFSH) and LH (cmLH) were evaluated in mid- and late-vitellogenic stage follicles by measuring the level of gonadal steroids, estradiol-17beta (Ε2) and 17,20beta-dihydroxy-4-pregnen-3-one (17,20beta-P). In addition, we evaluated the maturation-inducing potency of the GtHs on same stage follicles.</p> <p>Results</p> <p>Both cmFSH and cmLH significantly stimulated E2 production in mid-vitellogenic stage follicles. In contrast, only LH significantly stimulated the production of 17,20beta-P in late-vitellogenic stage follicles. Similarly, cmLH induced final oocyte maturation (FOM) in late-vitellogenic stage follicles.</p> <p>Conclusions</p> <p>Present results indicate that both FSH and LH may regulate vitellogenic processes, whereas only LH initiates FOM in chub mackerel.</p> | ||
| 546 | |a EN | ||
| 690 | |a Chub mackerel | ||
| 690 | |a Perciform | ||
| 690 | |a Asynchronous | ||
| 690 | |a FSH | ||
| 690 | |a LH | ||
| 690 | |a Steroidogenesis | ||
| 690 | |a Oocyte maturation | ||
| 690 | |a Gynecology and obstetrics | ||
| 690 | |a RG1-991 | ||
| 690 | |a Reproduction | ||
| 690 | |a QH471-489 | ||
| 655 | 7 | |a article |2 local | |
| 786 | 0 | |n Reproductive Biology and Endocrinology, Vol 10, Iss 1, p 71 (2012) | |
| 787 | 0 | |n http://www.rbej.com/content/10/1/71 | |
| 787 | 0 | |n https://doaj.org/toc/1477-7827 | |
| 856 | 4 | 1 | |u https://doaj.org/article/c58e0d77c9b04943a23fb18f1addf393 |z Connect to this object online. |