A Dataset for Constructing the Network Pharmacology of Overactive Bladder and Its Application to Reveal the Potential Therapeutic Targets of Rhynchophylline

<b>Objectives</b>: Network pharmacology is essential for understanding the multi-target and multi-pathway therapeutic mechanisms of traditional Chinese medicine. This study aims to evaluate the influence of database quality on target identification and to explore the therapeutic potentia...

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Váldodahkkit: Yan Tie (Dahkki), Jihan Liu (Dahkki), Yushan Wu (Dahkki), Yining Qiang (Dahkki), Ge'Er Cai'Li (Dahkki), Pingxiang Xu (Dahkki), Ming Xue (Dahkki), Liping Xu (Dahkki), Xiaorong Li (Dahkki), Xuelin Zhou (Dahkki)
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Almmustuhtton: MDPI AG, 2024-09-01T00:00:00Z.
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LEADER 00000 am a22000003u 4500
001 doaj_c603cf1184f84e788bbf6dd4262364a2
042 |a dc 
100 1 0 |a Yan Tie  |e author 
700 1 0 |a Jihan Liu  |e author 
700 1 0 |a Yushan Wu  |e author 
700 1 0 |a Yining Qiang  |e author 
700 1 0 |a Ge'Er Cai'Li  |e author 
700 1 0 |a Pingxiang Xu  |e author 
700 1 0 |a Ming Xue  |e author 
700 1 0 |a Liping Xu  |e author 
700 1 0 |a Xiaorong Li  |e author 
700 1 0 |a Xuelin Zhou  |e author 
245 0 0 |a A Dataset for Constructing the Network Pharmacology of Overactive Bladder and Its Application to Reveal the Potential Therapeutic Targets of Rhynchophylline 
260 |b MDPI AG,   |c 2024-09-01T00:00:00Z. 
500 |a 10.3390/ph17101253 
500 |a 1424-8247 
520 |a <b>Objectives</b>: Network pharmacology is essential for understanding the multi-target and multi-pathway therapeutic mechanisms of traditional Chinese medicine. This study aims to evaluate the influence of database quality on target identification and to explore the therapeutic potential of rhynchophylline (Rhy) in treating overactive bladder (OAB). <b>Methods</b>: An OAB dataset was constructed through extensive literature screening. Using this dataset, we applied network pharmacology to predict potential targets for Rhy, which is known for its therapeutic effects but lacks a well-defined target profile. Predicted targets were validated through in vitro experiments, including DARTS and CETSA. <b>Results</b>: Our analysis identified Rhy as a potential modulator of the M3 receptor and TRPM8 channel in the treatment of OAB. Validation experiments confirmed the interaction between Rhy and these targets. Additionally, the GeneCards database predicted other targets that are not directly linked to OAB, corroborated by the literature. <b>Conclusions</b>: We established a more accurate and comprehensive dataset of OAB targets, enhancing the reliability of target identification for drug treatments. This study underscores the importance of database quality in network pharmacology and contributes to the potential therapeutic strategies for OAB. 
546 |a EN 
690 |a overactive bladder 
690 |a network pharmacology 
690 |a rhynchophylline 
690 |a target identification 
690 |a Medicine 
690 |a R 
690 |a Pharmacy and materia medica 
690 |a RS1-441 
655 7 |a article  |2 local 
786 0 |n Pharmaceuticals, Vol 17, Iss 10, p 1253 (2024) 
787 0 |n https://www.mdpi.com/1424-8247/17/10/1253 
787 0 |n https://doaj.org/toc/1424-8247 
856 4 1 |u https://doaj.org/article/c603cf1184f84e788bbf6dd4262364a2  |z Connect to this object online.