miR-600 promotes ovarian cancer cells stemness, proliferation and metastasis via targeting KLF9

• Main Authors: Lili Shan (Author), Pingping Song (Author), Yangyang Zhao (Author), Na An (Author), Yanqiu Xia (Author), Yue Qi (Author), Hongyan Zhao (Author), Jing Ge (Author)
• Format: Book
• Published: BMC, 2022-05-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: 10.1186/s13048-022-00981-7; 1757-2215
• Keywords: Ovarian cancer; miR-600; KLF9; Stemness; Metastasis; Gynecology and obstetrics; RG1-991
• Source: Journal of Ovarian Research, Vol 15, Iss 1, Pp 1-10 (2022)

Abstract Previous studies have revealed that miRNAs participate in the pathogenesis of ovarian cancer; however, whether miR-600 is also involved remains unclear. In this study, we aimed to investigated the role of miR-600 in ovarian cancer progression. Here, miR-600 expression was significantly upregulated in ovarian cancer tissues and stem cells. Functional studies showed that miR-600 promoted ovarian cancer cell stemness, proliferation and metastasis. Mechanistic studies revealed that Kruppel like factor 9 (KLF9) was indicated as the target of miR-600. The luciferase reporter assay suggested that miR-600 directly bound to the 3'-untranslated region of KLF9. Additionally, miR-600 expression was negatively associated with KLF9 expression in human ovarian cancer tissues. Si-KLF9 partially abolished the discrepancy of self-renewal, growth and metastasis capacity between miR-600 knockdown ovarian cancer cells and control cells. In conclusion, our results suggest that miR-600 promotes ovarian cancer cell stemness, proliferation and metastasis via directly downregulating KLF9, and impairing miR-600 levels may be a new treatment strategy for ovarian cancer in the future.