iPseU-CNN: Identifying RNA Pseudouridine Sites Using Convolutional Neural Networks

• Main Authors: Muhammad Tahir (Author), Hilal Tayara (Author), Kil To Chong (Author)
• Format: Book
• Published: Elsevier, 2019-06-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: 2162-2531; 10.1016/j.omtn.2019.03.010
• Keywords: Therapeutics. Pharmacology; RM1-950
• Source: Molecular Therapy: Nucleic Acids, Vol 16, Iss , Pp 463-470 (2019)

Pseudouridine is the most prevalent RNA modification and has been found in both eukaryotes and prokaryotes. Currently, pseudouridine has been demonstrated in several kinds of RNAs, such as small nuclear RNA, rRNA, tRNA, mRNA, and small nucleolar RNA. Therefore, its significance to academic research and drug development is understandable. Through biochemical experiments, the pseudouridine site identification has produced good outcomes, but these lab exploratory methods and biochemical processes are expensive and time consuming. Therefore, it is important to introduce efficient methods for identification of pseudouridine sites. In this study, an intelligent method for pseudouridine sites using the deep-learning approach was developed. The proposed prediction model is called iPseU-CNN (identifying pseudouridine by convolutional neural networks). The existing methods used handcrafted features and machine-learning approaches to identify pseudouridine sites. However, the proposed predictor extracts the features of the pseudouridine sites automatically using a convolution neural network model. The iPseU-CNN model yields better outcomes than the current state-of-the-art models in all evaluation parameters. It is thus highly projected that the iPseU-CNN predictor will become a helpful tool for academic research on pseudouridine site prediction of RNA, as well as in drug discovery. Keywords: convolution neural network, deep learning, iPseU-CNN, pseudouridine sites, RNA