Impact of ghrelin on vitreous cytokine levels in an experimental uveitis model

• Main Authors: Turgut B (Author), Gul FC (Author), Dagli F (Author), Ilhan N (Author), Ozgen M (Author)
• Format: Book
• Published: Dove Medical Press, 2013-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: 1177-8881
• Keywords: Therapeutics. Pharmacology; RM1-950
• Source: Drug Design, Development and Therapy, Vol 2013, Iss default, Pp 19-24 (2013)

Burak Turgut,1 Fatih Cem G&uuml;l,1 Ferda Dağli,2 Nevin Ilhan,3 Metin &Ouml;zgen41Department of Ophthalmology, School of Medicine, Firat University, Elazig, 2Department of Pathology, School of Medicine, İn&ouml;n&uuml; University, Malatya, 3Department of Biochemistry, School of Medicine, Firat University, Elazig, 4Department of Rheumatology, School of Medicine, İn&ouml;n&uuml; University, Malatya, TurkeyBackground: The purpose of this study was to investigate the effect of intraperitoneal ghrelin on vitreous levels of interleukin (IL)-1, IL-6, and tumor necrosis factor-alpha (TNF-&alpha;) and to compare its effects with those of intraperitoneal infliximab in an experimental uveitis model.Methods: Twenty-four male rats were assigned to four groups of six rats in each. All the rats, except for those in group 1 (controls), were injected intravitreally with concanavalin A to induce experimental uveitis. Rats in group 2 (sham) were not given any treatment after uveitis was induced. Rats in group 3 were given intraperitoneal infliximab 0.5 mg/100 mL on days 0, 1, 3, 5, and 7 following induction of uveitis on day 14 of the study. Rats in group 4 were given intraperitoneal ghrelin 10 ng/kg/day for 7 days following induction of uveitis. On day 21 of the study, enucleated globes were subjected to histopathologic examination. Vitreous levels of IL-1, IL-6, and TNF-&alpha; were measured by enzyme-linked immunosorbent assay.Results: Vitreous levels of IL-1, IL-6, and TNF-&alpha; were significantly increased in the sham group relative to the control group (P < 0.05), but showed a significant decrease in the group treated with infliximab (P < 0.05). Cytokine levels also decreased in the ghrelin-treated group, but the decrease was not statistically significant (P > 0.05).Conclusion: Ghrelin failed to decrease the IL-1, IL-6, and TNF-&alpha; levels that play a critical role in the pathogenesis of uveitis.Keywords: uveitis, tumor necrosis factor-alpha, interleukin-1, interleukin-6, infliximab, ghrelin