The Gut of Healthy Infants in the Community as a Reservoir of ESBL and Carbapenemase-Producing Bacteria

• 主要な著者: Ali F. Saleem (著者), Ahreen Allana (著者), Lauren Hale (著者), Alondra Diaz (著者), Raul Salinas (著者), Cristina Salinas (著者), Shahida M. Qureshi (著者), Aneeta Hotwani (著者), Najeeb Rahman (著者), Asia Khan (著者), Anita K. Zaidi (著者), Patrick C. Seed (著者), Mehreen Arshad (著者)
• フォーマット: 図書
• 出版事項: MDPI AG, 2020-05-01T00:00:00Z.
• 主題: article
• オンライン･アクセス: Connect to this object online.

要約

The recent rapid rise of multi-drug resistant Enterobacteriaceae (MDR-E) is threatening the treatment of common infectious diseases. Infections with such strains lead to increased mortality and morbidity. Using a cross-sectional study, we aimed to estimate the prevalence of gut colonization with extended spectrum beta-lactamase (ESBL) producing Enterobacteriaceae among healthy infants born in Pakistan, a setting with high incidence of MDR-E infections. Stool samples were collected from 104 healthy infants between the ages of 5 and 7 months. Enterobacteriaceae isolates were screened for resistance against several antimicrobial classes. Presence of ESBL and carbapenemase genes was determined using multiplex PCR. Sequence types were assigned to individual strains by multi-locus sequence typing. Phylogenetic analysis of <i>Escherichia coli</i> was done using the triplex PCR method. Forty-three percent of the infants were positive for ESBL-producing Enterobacteriaceae, the majority of which were <i>E. coli</i>. We identified several different ESBL <i>E. coli</i> sequence types most of which belonged to the phylogenetic group B2 (23%) or D (73%). The widespread colonization of infants in a developing country with ESBL-producing Enterobacteriaceae is concerning. The multiple sequence types and reported non-human sources support that multiple non-epidemic MDR lineages are circulating in Pakistan with healthy infants as a common reservoir.

記述事項

10.3390/antibiotics9060286
2079-6382