Evaluation and mechanism study of Pien Tze Huang against EV-A71 infection

• Main Authors: Huiqiang Wang (Author), Fenbei Chen (Author), Shicong Wang (Author), Yuhuan Li (Author), Ting Liu (Author), Yinghong Li (Author), Hongbin Deng (Author), Jingwen Dong (Author), Jing Pang (Author), Danqing Song (Author), Dousheng Zhang (Author), Juan Yu (Author), Yanxiang Wang (Author)
• Format: Book
• Published: Frontiers Media S.A., 2023-10-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

Hand, foot, and mouth disease (HFMD) caused by enterovirus A71 (EV-A71) infection, currently lacks specific preventive and therapeutic interventions. Here, we demonstrated that Pien Tze Huang (PZH) could dose-dependently inhibit EV-A71 replication at the cellular level, resulting in significant reductions in EV-A71 virus protein 1 (VP1) expression and viral yields in Vero and human rhabdomyosarcoma cells. More importantly, we confirmed that PZH could protect mice from EV-A71 infection for the first time, with Ribavirin serving as a positive control. PZH treatment reduced EV-A71 VP1 protein expression, viral yields in infected muscles, and improved muscle pathology. Additionally, we conducted a preliminary mechanism study using quantitative proteomics. The results suggested that the suppression of the PI3K/AKT/mTOR and NF-κB signaling pathways may contribute to the anti-EV-A71 activity of PZH. These findings provide strong evidence supporting the potential therapeutic application of PZH for EV-A71 infection management.

Item Description

1663-9812
10.3389/fphar.2023.1251731