MicroRNA-552 expression in colorectal cancer and its clinicopathological significance

• Main Authors: Joon Im (Author), Soo Kyung Nam (Author), Hye Seung Lee (Author)
• Format: Book
• Published: Korean Society of Pathologists & the Korean Society for Cytopathology, 2021-03-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: 2383-7837; 2383-7845; 10.4132/jptm.2021.01.17
• Keywords: mir-552; colorectal neoplasms; p53; pten; prognosis; Pathology; RB1-214
• Source: Journal of Pathology and Translational Medicine, Vol 55, Iss 2, Pp 125-131 (2021)

Background MicroRNA-552 (miR-552) has been reported to correlate with the development and progression of various cancers, including colorectal cancer (CRC). This study aimed to investigate miR-552 expression in cancer tissue samples compared to normal mucosal tissue and its role as a diagnostic or prognostic marker in CRC patients. Methods Normal mucosal tissues and primary cancer tissues from 80 surgically resected CRC specimens were used. Quantitative real-time polymerase chain reaction was performed for miR-552 and U6 small nuclear RNA to analyze miR-552 expression and its clinicopathological significance. Immunohistochemistry for p53 and phosphatase and tension homolog (PTEN) was performed to evaluate their association with miR-552 expression. Results miR-552 expression was significantly higher in primary cancer tissues compared to normal mucosal tissues (p<.001). The expression level of miR552 was inversely correlated with that of PTEN (p=.068) and p53 (p=.004). Survival analysis showed that high miR-552 expression was associated with worse prognosis but this was not statistically significant (p=.255). However, patients with CRC having high miR-552 expression and loss of PTEN expression had significantly worse prognosis than others (p=.029). Conclusions Our results suggest that high miR-552 expression might be a potential diagnostic biomarker for CRC, and its combined analysis with PTEN expression can possibly be used as a prognostic marker.