Poor Sensitivity of the MALDI Biotyper^® MBT Subtyping Module for Detection of <i>Klebsiella pneumoniae</i> Carbapenemase (KPC) in <i>Klebsiella</i> Species

• Main Authors: Luz Cuello (Author), Judith Alvarez Otero (Author), Kerryl E. Greenwood-Quaintance (Author), Liang Chen (Author), Blake Hanson (Author), Jinnethe Reyes (Author), Lauren Komarow (Author), Lizhao Ge (Author), Zane D. Lancaster (Author), Garrett G. Gordy (Author), Audrey N. Schuetz (Author), Robin Patel (Author)
• Format: Book
• Published: MDPI AG, 2023-09-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

Rapid detection of <i>Klebsiella pneumoniae</i> carbapenemase (KPC) in the <i>Klebsiella</i> species is desirable. The MALDI Biotyper^® MBT Subtyping Module (Bruker Daltonics) uses an algorithm that detects a peak at ~11,109 m/z corresponding to a protein encoded by the <i>p019</i> gene to detect KPC simultaneously with organism identification by a matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-ToF MS). Here, the subtyping module was evaluated using 795 clinical <i>Klebsiella</i> isolates, with whole genome sequences used to assess for <i>bla</i>_KPC and <i>p019</i>. For the isolates identified as KPC positive by sequencing, the overall sensitivity of the MALDI-ToF MS subtyping module was 239/574 (42%) with 100% specificity. For the isolates harboring <i>p019</i>, the subtyping module showed a sensitivity of 97% (239/246) and a specificity of 100%. The subtyping module had poor sensitivity for the detection of <i>bla</i>_KPC-positive <i>Klebsiella</i> isolates, albeit exhibiting excellent specificity. The poor sensitivity was a result of <i>p019</i> being present in only 43% of the <i>bla</i>_KPC-positive <i>Klebsiella</i> isolates.

Item Description

10.3390/antibiotics12091465
2079-6382